Advances in Analytical Chemistry

Advances in Analytical Chemistry is a peer-reviewed research journal that is devoted to the dissemination of new and original knowledge in all branches of analytical chemistry. The journal publishes features and news articles about major advances, trends, and challenges in analytical chemistry. These articles include peer-reviewed features written by researchers and news stories that discuss novel analytical concepts and instruments.

ICV 2015: 76.84; ICV 2016: 84.50
Editor-in-chief: Syed Rizvi
p-ISSN: 2163-2839
e-ISSN: 2163-2847


Forthcoming Issue:

Method Development and Validation of Carisoprodol and its Impurities by Ultra Violet-High Performance Liquid Chromatography
Author(s): T. Rohith, S. Ananda, Netkal M. Made Gowda
Abstract: Ultraviolet-high performance liquid chromatographic (UV-HPLC) method was developed for the quantitation of carisoprodol and its impurities viz 2-methyl-2-propylpropane-1, 3-diyl dicarbamate (impurity-D) and N-isopropyl -2-methyl-2-propyl-3-hydroxy propyl carbamate (impurity-B) in active pharmaceutical ingredient. Validation of the method showed excellent sensitivity, selectivity, accuracy, precision and ruggedness. Efficient chromatographic separation was achieved on a Zorbax eclipse XDB C (8) 250 mm X 4.6 mm id, 5┬Ám , stationary phase in gradient mode and quantitation by ultraviolet wavelength detection. The method was validated as per International Conference of Harmonization (ICH) guidelines in terms of Quantitation limit (QL), Detection limit (DL), Linearity, Precision, Accuracy and Specificity. The QL and DL values for impurity-B were found to be 0.015% and 0.008% and for that of impurity-D were found to be 0.26% and 0.13%respectively, with respect to sample concentration. The method was linear within the range of QL to 200% for the two impurities. Thus, the newly developed method was found to be accurate, efficient and stable. The characterization of these impurities was carried out for the confirmation of respective structures using nuclear magnetic resonance spectroscopy (NMR).