Research In Cancer and Tumor

International Journal of Cancer and Tumor is a peer-reviewed international journal which provides a forum for prompt communication of original and innovative research findings that have relevance to understanding the etiology of cancer and to improving the treatment and survival of patients.

Shen Hu

Editorial Board Member of Research In Cancer and Tumor

Associate Professor, University of California, Los Angeles, School of Dentistry & Jonsson Comprehensive Cancer Center, USA

Research Areas

Cancer Proteomics, Cancer Metabolomics, Single Cell Proteomics, Cancer Stem Cell Biology, Bioanalytical Chemistry

Experience

2011-presentTenured Associate Professor, UCLA School of Dentistry, UCLA Comprehensive Cancer Center
2004-2011Assistant Professor, UCLA School of Dentistry, UCLA Comprehensive Cancer Center
2001-2003Research Assistant Professor, University of Washington, Seattle, WA

Publications: Conferences/Workshops/Symposiums/Journals/Books

[1]  Martha Arellano, Roger Li , Xiaojun Liu, Yongmin Xie, Xinmin Yan, Joseph Loo, Shen Hu. Identification of tetranectin as a potential biomarker for metastatic oral cancer. Int J Mol Sci. 2010, 11(9): 3106-21.
[2]  Yi Liu, Wang L, Zhou X, Shen Hu, Zhang S, Wu H. Effect of the antimicrobial decapeptide KSL on the growth of oral pathogens and Streptococcus mutans biofilm. Int J Antimicrob Agents. 2011; 37(1): 33-8.
[3]  Xiaojun Liu, Min Zhang, Bill Go, Shen Hu. Membrane proteomic analysis of pancreatic cancer cells. J Biomed Sci. 2010;17:74.
[4]  Yi Liu, Diana Messadi, Wu H, Shen Hu. Oral lichen planus is a unique disease model for studying chronic inflammation and oral cancer. Med Hypotheses. 2010, 75(6):492-4.
[5]  Shen Hu, Arjan Vissink, et al. Identification of autoantibody biomarkers for primary Sjögren's syndrome using protein microarrays. Proteomics, 2011, 11(8):1499-507
[6]  Shen Hu, Kai Gao, et al. Validation of protein biomarkers for primary Sjögren's syndrome. Arthritis Care Research, 2010;62(11):1633-8
[7]  Shen Hu, David T. Wong. Lectin microarray. Proteomics/Clinical Applications, 2009, 3:148–154
[8]  Shen Hu, Michael Zhou, et al. Systems analysis of Sjögren's syndrome and mucosa-associated lymphoid tissue (MALT) lymphoma. Arthritis & Rheumatism, 2009, 60(1): 81-92
[9]  Kai Gao, Hui Zhou, Lei Zhang, Jin Wook Lee, Qing Zhou, Shen Hu, Lawrence E. Wolinsky, et al. Systemic disease-induced salivary biomarker profiles in mouse models of melanoma and non-small cell lung cancer. PLoS One. 2009, 4(6): e5875
[10]  Fang Wei, Prabhudas Patel, Wei Liao, Kishore Chaudhry, Lei Zhang, Martha Arellano, Shen Hu, et al. Electrochemical sensor for multiplex biomarkers detection. Clinical Cancer Research 2009, 15(13): 4446-52
[11]  Martha Arellano, Jiang Jiang, Xiaofeng Zhou, Lifeng Zhang, Hui Ye, David Wong, Shen Hu. "Current advances in identification of cancer biomarkers in saliva". Frontiers in Bioscience, 2009, 1:296-303
[12]  Shen Hu, Jiang Jiang, et al. "Analysis of saliva proteins by 2-D gel electrophoresis and Western blotting". Methods in Molecular Biology. Edited by M. Cullinan et al. Humana Press, 2009
[13]  Jiang Jiang, Nojin Park, Shen Hu, et al. A universal pre-analytic solution for concurrent stabilization of salivary proteins, RNA and DNA at ambient temperature. Arch Oral Biology 2009, 54(3): 268-73
[14]  Weihong Yan, Rolf Apweiler, Brian M. Balgley, Pinmanee Boontheung, Jonathan L. Bundy, Benjamin J. Cargile, Steve Cole, Xueping Fang, Mireya Gonzalez-Begne, Timothy J. Griffin, Fred Hagen, Shen Hu, et al. Systematic comparison of the human saliva and plasma proteomes. Proteomics Clinical Applications 2009, 3(1): 116-134
[15]  Reuben H. Kim, Roy Kim, Wei Chen, Shen Hu, Ki-Hyuk Shin, No-Hee Park and Mo K. Kang. Association of hsp90 to the hTERT promoter is necessary for hTERT expression in human oral cancer cells. Carcinogenesis. 2008, 29(12): Scientific & Academic Publishing http://www.sapub.org 2425-31
[16]  Shen Hu, Martha Arellano, et al. Salivary proteomics for oral cancer biomarker discovery. Clinical Cancer Research, 2008, 14(19): 6246-52 17.Martha Arellano, Shen Hu, et al. Multiplexed immunobead-based assay for detection of oral cancer protein biomarkers in saliva. Oral Diseases, 2008, 14(8): 705-12 18 Lifeng Zhang, Jiang Jiang, David Wong, Shen Hu. Quantitative proteomic analysis of serum proteins from metastatic oral cancer patients. The Open Proteomics Journal, 2008, 1: 72-78
[17]  Martha Arellano, Shen Hu, et al. Multiplexed immunobead-based assay for detection of oral cancer protein biomarkers in saliva. Oral Diseases, 2008, 14(8): 705-12
[18]  Lifeng Zhang, Jiang Jiang, David Wong, Shen Hu. Quantitative proteomic analysis of serum proteins from metastatic oral cancer patients. The Open Proteomics Journal, 2008, 1: 72-78
[19]  Hui Ye, Anxun Wang, Bao-shiang Lee, Tianwei Yu, Shihu Shen, Tingsheng Peng, Shen Hu, David L. Crowe, Xiaofeng Zhou. "Proteomic based identification of manganese superoxide dismutase 2 (SOD2) as a metastasis marker for oral squamous cell carcinoma". Cancer Genomics & Proteomics, 2008, 5: 85-94
[20]  Paul Denny, Fred K. Hagen, Markus Hardt, Lujian Liao, Weihong Yan, Martha Arellanno, Sara Bassilian, Gurrinder S. Bedi, Pinmannee Boontheung, Daniel Cociorva, Claire M. Delahunty, Trish Denny, Jason Dunsmore, Kym F. Faull, Joyce Gilligan, Mireya Gonzalez-Begne, Frédéric Halgand, Steven C. Hall, Xuemei Han, Bradley Henson, Johannes Hewel, Shen Hu, et al. The proteomes of human parotid and SM/SL gland salivas collected as the ductal secretions. Journal of Proteome Research, 2008, 7: 1994-2006
[21]  Shen Hu, Jianghua Wang, et al."Salivary proteomic and genomic biomarkers for primary Sjögren's Syndrome". Arthritis & Rheumatism, 2007, 56 (11), 3588–600
[22]  Shen Hu, David Wong. "Oral cancer proteomics". Current Opinion in Molecular Therapeutics, 2007 9(5): 467-76
[23]  Shen Hu, Tianwei Yu, et al. "Discovery of oral fluid biomarkers for human oral cancer using mass spectrometry". Cancer Genomics and Proteomics, 2007, 4(2): 55-64
[24]  Shen Hu, Yun Yen, et al. "Implications of salivary proteomics in drug discovery and development: a focus on cancer drug discovery". Drug Discovery Today, 2007, 12, 911-916
[25]  Jianghua Wang, Shen Hu, et al. "Salivary biomarkers for the detection of primary Sjögren's Syndrome". Future Rheumatology, 2007, 2(5), 447-449
[26]  Shen Hu, et al. "Human saliva proteome analysis and disease biomarker discovery". Expert Review of Proteomics, 2007, 4(4): 531-8
[27]  Shen Hu, et al. "Human saliva proteome analysis", Annals of the New York Academy of Sciences, 2007, 1098:323-9
[28]  Shen Hu, et al. "Human body fluid proteomics", Proteomics, 2006, 6(23): 6326-53 (most accessed article 10/2006–9/2007)
[29]  Shen Hu, Yang Li, et al "Human saliva proteome and transcriptome". Journal of Dental Research, 2006, 85(12): 1129-33 Scientific & Academic Publishing http://www.sapub.org
[30]  Shen Hu, Yongming Xie, et al. "Large-scale identification of human oral fluid proteins by liquid chromatography/mass spectrometry and 2-D gel electrophoresis/mass spectrometry". Proteomics, 2005, 5(6): 1714-28
[31]  Shen Hu, Patricia Denny, et al. "Differential expressed protein markers in submandibular and sublingular glandular secretions". International Journal of Oncology, 2004, 25(5): 1423-30
[32]  Xiaofeng Zhou, Steven W. Cole, Shen Hu, David Wong. "Detection of DNA copy number abnormality by microarray expression analysis". Human Genetics, 2004, 114: 464-7
[33]  David Michels, Shen Hu, Michael Eggertson, Amy Dambrawitcz, Norman J. Dovichi. "CSE-MECC fully automated two-dimensional capillary electrophoresis analysis of Deinococcus radiodurans protein homogenate". Electrophoresis, 2004, 25: 3098-105
[34]  Shen Hu, David Michels, Chootima Ratisoontorn, Michael Cunningham, Norman J. Dovichi. "Capillary sieving electrophoresis/micellar electrokinetic capillary chromatography for two-dimensional protein fingerprinting of single mammalian cells". Analytical Chemistry, 2004, 76: 4044-9
[35]  Mingliang Ye, Shen Hu, Norman J. Dovichi. "On-line protein digestion and peptide mapping by capillary electrophoresis with post-column labeling for laser induced fluorescence detection". Electrophoresis. 2004, 25: 1319-26
[36]  Mingliang Ye, Shen Hu, Wes W.C. Quigley, Norman J. Dovichi. "Post-column fluorescence derivatization of proteins and peptides in capillary electrophoresis with a sheath flow reactor and 488 nm argon ion laser excitation". Journal of Chromatography A, 2004, 1022, 201-6
[37]  Norman J. Dovichi and Shen Hu. "Chemical Cytometry". Current Opinion of Chemical Biol ogy, 2003, 7: 603-8
[38]  Shen Hu, Le Zhang, Rick Newitt, Ruedi Aebersold, Jim Kraly, Megan Jones, and Norman J. Dovichi. "Identification of proteins in single-cell capillary electrophoresis fingerprints based on co-migration with standard proteins". Analytical Chemistry, 2003, 75, 3502-5
[39]  Shen Hu, Le Zhang, Sergey Krylov, and Norman J. Dovichi. "Cell-cycle dependent protein fingerprint from a single cancer cell: image cytometry coupled with single-cell capillary sieving electrophoresis". Analytical Chemistry, 2003, 75, 3495-501
[40]  David Michels, Shen Hu, Regine Schoenherr, Mike Eggertson, Norman Dovichi, "Fully automated two-dimensional capillary electrophoresis for high-sensitivity protein analysis". Molecular and Cellular Proteomics, 2002, 1, 69-74
[41]  Shen Hu and Norman Dovichi. "Capillary Electrophoresis for the Analysis of Biopolymers". Analytical Chemistry, 2002, 74, 2833-50
[42]  Shen Hu, Jiang Jiang, Lillian Cook, Dawn Richards, Laura Horlick, Brian Wong, and Norman Dovichi. "Capillary SDS-DALT electrophoresis with laser-induced fluorescence for size-based analysis of proteins in human colon cancer cells". Electrophoresis, 2002, 18: 3136-42 Scientific & Academic Publishing http://www.sapub.org
[43]  Le Zhang, Shen Hu, Lillian Cook, and Norman Dovichi. "Analysis of Aminophospholipid Molecular Species by Methyl-beta-cyclodextrin Modified Micellar Electrokinetic Capillary Chromatography with Laser-Induced Fluorescence Detection". Electrophoresis, 2002, 18: 3071-7
[44]  Shen Hu, Mingliang Ye, Gloria Surh, John I. Clark, Norman Dovichi. "Analysis of proteins by capillary SDS-DALT electrophoresis with laser-induced fluorescence detection". LC-GC Europe, 2002, 3, 166-71
[45]  Shen Hu, Sergey Krylov, Le Zhang, Zheru Zhang, Rebecca Lee, Norman Dovichi. "Molecular cytometry: analysis of proteins in single cells". Proceeding of the International Society for Optical Engineering (SPIE 2002), 2002, Vol. 4625, 98-105
[46]  Shen Hu, Amy Dambrowitz, David Michels, Le Zhang, and Norman Dovichi. "The single cell proteome project". Radiation Research, 2001, 156, 444-5 47.Shen Hu, Le Zhang, Lillian Cook, Norman Dovichi. "Capillary SDS-DALT electrophoresis of p roteins in a single human cancer cell". Electrophoresis, 2001, 22, 3677-82
[47]  Shen Hu, Le Zhang, Norman Dovichi. "Characterization of the interaction between phospholipids and proteins by capillary electrophoresis with laser induced fluorescence detection". Journal of Chromatography A, 2001, 924, 369-75
[48]  Shen Hu, Paul C. H. Li, "A capillary zone electrophoretic method for the study of formation of a covalent conjugate between microcystin LR and protein phosphatase 2A". Analyst, 2001, 126, 1001-4
[49]  Shen Hu, Rebecca Lee, Zheru Zhang, Sergey N. Krylov, Norman Dovichi. "Protein analysis of an individual Caenorhabditis elegans single-cell embryo by capillary electrophoresis". Journal of Chromatography B, 2001, 752, 307-10
[50]  Shen Hu, Zheru Zhang, Lillian Cook, Eric Carpenter, Norman Dovichi. "Separation of proteins by sodium dodecyl sulfate-capillary electrophoresis in hydroxypropyl cellulose sieving matrix with laser-induced fluorescence detection". Journal of Chromatography A, 2000, 894, 291-6
[51]  Le Zhang, Sergey Krylov, Shen Hu, Norman Dovichi. "Methyl-b-cyclodextrin modified micellar electrokinetic capillary chromatography with laser-induced fluorescence for separation and detection of phospholipids". Journal of Chromatography A, 2000, 894, 129-34
[52]  Shen Hu, Min Zhang, Daiwen Pang, Jieke Cheng. "Powder microelectrodes for capillary electrophoresis with ampeormetric detection". Analytical Science, 2000, 16, 807-10
[53]  Shen Hu, Paul C. H. Li. "Micellar electrokinetic capillary chromatographic separation and fluorescent detection of amino acids derivatized with 4-fluoro-7-nitro-2, 1, 3-benzoxadiazole". Journal of Chromatography A, 2000, 876, 183-91
[54]  Shen Hu, Enqin Fu, Paul C. H. Li. "Capillary electrophoretic separation enhanced by a macrocyclic dioxopolyamine additive". Journal of Chromatography A, 1999, 844, 439-46
[55]  Yuandi Zhao, Daiwen Pang, Shen Hu, Zongli Wang, Jieke Cheng, Hongping Dai. "DNA-modified electrodes. Part Scientific & Academic Publishing http://www.sapub.org
[56]  Optimization of covalent immobilization of DNA on self-assembled monolayers". Talanta, 1999, 49, 751-6
[57]  Yuandi Zhao, Daiwen Pang, Shen Hu, Zongli Wang, Jieke Cheng, Yipeng Qi, Hongping Dai, Bingwei, Mao, Zhonfqun Tian, Jin Luo, Zhong Hua, Lin. "DNA-modified electrodes. Part 3. Spectroscopic characterization of DNA-modified gold electrodes". Analytica Chimica Acta, 1999, 388, 93-101
[58]  Paul C H Li, Shen Hu, and Paul Lam. Development of a capillary zone electrophoretic method for the rapid separation and detection of hepatotoxic microcystins. Marine Pollution Bulletin, 1999, 39: 250-4
[59]  Le Zhang, Hong Chen, Shen Hu, Jieke Cheng, Zhiwang Li, Mei Shao. "Determination of the amino acid neurotransmitters in the dorsal root ganglion of the rat by capillary electrophoresis with laser-induced fluorescence and charge-coupled device". Journal of Chromatography B, 1998, 707, 59-67
[60]  Shen Hu, Zongli Wang, Peibiao Li, Jieke Cheng. "Amperometric detection in capillary electrophoresis with etched joint". Analytical Chemistry, 1997, 69: 264-7
[61]  Jeff Brumbaugh, Robert Ferris, Shen Hu. "Head and Neck Cancer Virology". Head and Neck Cancer: Multimodality Management. Edited by Jacques Bernier, Springer Science, 2010, in press
[62]  Xiaofeng Zhou, Shen Hu, David T. Wong. "High throughput molecular profiling approaches for the identifications of genomic alterations and therapeutic targets in oral cancer". Signaling Pathways in Squamous Cancer, Edited by Carter van Waes and Adam Glick, Springer Science, 2010, in press
[63]  Shen Hu, Xiaofeng Zhou, Mike Hu, Bernie Zimmerman, David Wong. "The role of high throughput molecular analysis of biofluids and tumors in patients with oral cancer". Oral Cancer Metastasis. Edited by Jeffrey Myers, Springer Science, 2010, pp. 105-120
[64]  Shen Hu. "Proteomics and Related Applications". Informatics in Oral Medicine: Advanced Techniques in Clinical and Diagnostic Technologies. Edited by Andriani Daskalaki, Medical Info Science, Berlin, 2009, pp. 17-29
[65]  Shen Hu, Markus Hardt, Susan Fisher, David Wong, John Yates, James Melvin, Joseph Loo. "Human salivary proteomics". Salivary Diagnostics. Edited by David Wong, Blackwell Publishing, November 2008, pp. 122-128
[66]  Norman J. Dovichi, Shen Hu, David Michels, Danqian Mao, and Amy Dambrowitz. "Single cell proteomics". In: Proteomics: the Catalyst to Biological Discovery, Edited by Tim Veenstra & John Yates, John Wiley and Sons Inc., 2006, pp. 226-245
[67]  Norman Dovichi, Shen Hu, David Michels, Zheru Zhang, and Sergey Krylov. "Proteome analysis by capillary electrophoresis". In: Biotechnology, 2nd Edition, Vol. 5b Genomics and Bioinformatics, Edited by Christopher Sensen, Wiley-VCH, Weinheim, 2001, pp. 269-278