Research In Cancer and Tumor

International Journal of Cancer and Tumor is a peer-reviewed international journal which provides a forum for prompt communication of original and innovative research findings that have relevance to understanding the etiology of cancer and to improving the treatment and survival of patients.

Alain Thierry

Editorial Board Member of Research In Cancer and Tumor

Staff Scientist, Sysdiag UMR3145-CNRS, France

Research Areas

Cancer, Diagnostic, DNA, Therapy

Education

2003HabilitationHabilitation à Diriger les Recherches, Biology-Health, Univ. Montpellier II
1987Certificat d'Etudes SupérieuresHuman Biology in Clinical and Experimental Oncology, Faculté de Médecine Paris-Sud
1986Ph.DCellular and Molecular Pharmacology, Université Montpellier 2
1983M.ScCellular and Molecular Biology, Université de Clermont-Fd II
1983Diplôme d'IngénieurUniversité de Clermont-Fd II
1982B.ScUniversité de Clermont

Experience

2001-2007Associate Professor, Université des Sciences, Montpellier 2
2003-2004Board Member and Director R&D, MedinCell SA, Montpellier, France
1997-2000Scientific Director, Department of Gene Therapy and Delivery, Biovector Therapeutics, Toulouse, France
1996-1997Group Leader, Transgene SA, Département of Biochemistry, Strasbourg, France
1992-1995Visiting Scientist, Laboratory of Tumor Cell Biology, R.C. Gallo Chief, Director National Cancer Institute, National Institutes of Health, Bethesda, USA
1992-1994Adjunct Assistant Professor, Department of Radiation Medicine, Georgetown University School of Medicine, Lombardi Cancer Institute, Washington DC, USA
1988-1992Postdoctoral Fellow, Department of Radiation Medicine, Lombardi Cancer Centre, Georgetown University, Washington DC, USA
1986-1988Postdoctoral Fellow, Centre Anticancereux Jean Perrin, INSERM U71, Clermont-Fd, France

Publications: Journals

[1]  Vignon, J., Privat, A. , Chaudieu, I., Thierry, A., Kamenka, J.M., and Chicheportiche, R. : [3H] thienyl-phencyclidine binds to two different sites in rat brain. Brain Res. 378 : 133-141, 1986
[2]  Madelmont, J.C., Godeneche, B., Labarre, P., Thierry, A., and Veyre, A. : Comparative study of the efficiency of 2-chloroethyl and 2-fluoroethyl nitrosoureas. Eur. J. Med. Chem. 22 : 261-264, 1987
[3]  Thierry, A. and Chicheportiche, R. : Use of ATP bioluminescence measurements for the estimation of biomas during biological humification. Appl. Microbiol Biotechnol. 28 : 199-202, 1988
[4]  Thierry, A. Doly, M., Braquet, P., Cluzel, J., and Meyniel, G.: Presence of specific platelet-activating factor binding sites in the rat retina. Eur. J. Pharmacol. 163 : 97-101, 1989
[5]  Thierry, A., Jorgensen, T., Forst, D., Belli, J.A.., Dritschilo, A., and Rahman, A. : Modulation of multidrug resistance in Chinese hamster cells by liposome-encapsulated doxorubicin. Cancer Commun. 1 : 311-316, 1989.
[6]  Godeneche, D., Rapp, M. Thierry, A., Laval, F., Madelmont, J.C., Chollet, P. and Veyre, A. : DNA damage induced by a new chloroethyl nitrosourea on malignant melanoma cells. Cancer Res. 50 : 5898-5903, 1990.
[7]  Thierry, A.R., Dritschilo, A., and Rahman, A. : Liposomal carrier system as a novel approach to transport ologonucleotides into cells. Proc. Am. Assoc. Cancer Res. 31/2642, 1990
[8]  Oudard, S., Thierry, A., Jorgensen, T.J., and Rahman, A : Senzitization of multidrug-resistant colon cancer cell lines to doxorubicin encapsulated in liposome. Cancer Chemother. Pharmacol. 28: 259-265, 1991
[9]  Thierry, A.R., and Dritschilo, A.: Intracellular avaibility of unmodified, phosphorothioated and liposomally encapsulated oligodeoxynucleotides for antisense activity. Nucleic Acids Res. 21: 5691-5698, 1992
[10]  Thierry, A.R., Dritschilo, A. and Rahman, A : Effect of liposomes on P-glycoprotein function in multidrug resistance cells. Biochem. Biophys. Res. Commun. 187 : 1098-1105, 1992
[11]  Thierry, A.R., and Dritschilo, A. : Liposomal delivery of antisense oligodeoxynucleotides : Application to the modulation of multidrug resistance in cancer cells. Ann. NY Acad. Sci. 660 : 300-302, 1992
[12]  Thierry, A.R. Rahman, A. , and Dritschilo, A. : Overcoming multidrug resistance in human tumor cells using free and liposomally encapsulated antisense oligodeoxynucleotides. Biochem. Biophys. Res. Commun. 190 : 952-960, 1993
[13]  Thierry, A.R., Bradley, M.O., and Chrisey, L.A.: Progress in antisense research and development. Antisense Res. Dev. 3 : 411-416, 1993
[14]  Thierry, A.R., Vige, D., Belli, J.A., Dritschilo, A. , and Rahman, A. : Modulation of doxorubicin resistance in multidrug resistance cancer cells by liposomes. FASEB. J. 7572-579, 1993
[15]  Rahman, A., Thierry, A.R., Husain, S.R., and Dritschilo, A.: Clinical evaluation of liposome encapsulated doxorubicin and the modulation of multidrug resistance in cancer cells. J. Liposome Res. 4 : 167-192, 1994
[16]  Lisziewicz, J. Sun, D. Weichold, F.F., Thierry, A.R., Lusso, P. Tang, J. Gallo, R.C., and Agrawal, S. : Antisense oligodeoxynucleotide phosphorothioate complementary to Gag mRNA blocks replication of human immunodeficiency virus type 1 in human peripheral blood cells; Proc. Natl. Acad. Sci. USA 91, 7942-7946, 1994
[17]  Thierry, A.R., Bryant, J.L., Aksentijevich, I. et al : Transgene expression in mice using a new liposomal delivery system. AIDS Res. Hum. Retrov. 10, sup 3, 1994.
[18]  Lunardi-Iskandar, Y., Bryant, J.L., Zeman, R.A., Lam, V.H. Samaniego, F. Besnier, J.M., Hermans, P. Thierry, A.R., Gill, P., and Gallo, R.C.: Tumorigenesis and metastasis of neoplastic Kaposi's sarcoma cell line in immonodeficient mice blocked by an human pregnancy hormone. Nature 375 : 64-68, 1995.
[19]  Thierry, A.R., Rahman, A., and Dritschilo, A. : A new procedure for the preparation of liposomal doxorubicin : biological activity in multidrug-resistant tumor cells. Cancer Chemother. Pharmacol. 35, 84-88, 1994.
[20]  Thierry, A.R., Zeman, R.A., Hunh, J.W., Bryant, J.L., Gallo, R.C. and Lunardi-Iskandar, Y. : In vitro and in vivo inhibition of tumorigenicity of neoplastic Kaposi's sarcoma cell line (KS Y-1) by liposomal IL-6, IL-8 and VEGF antisense oligodeoxynucleotides. Proc. Am. Assoc. Cancer. Res. 36, 413, 1995.
[21]  Thierry, A.R., Lunardi-Iskandar, Y, Bryant, J.L., Rabinovitch, P., Gallo, R.C., and Mahan, L.C. : Systemic gene therapy : biodistribution and long-term expression of a transgene in mice. Proc. Natl. Acad. Sci. USA. 92, 9742-9746, 1995.
[22]  Aksentijevish, I. Pastan, Y. Lunardi-Iskandar, R. C. Gallo, M. M. Gottesman, and A. R. Thierry. In vitro and in vivo liposome-mediated gene transfer leads to human MDR1 expression in mouse bone marrow progenitor cells. Human Gene Ther. 7:1111-1122, 1996.
[23]  M. Baudard, T.R. Flotte, J.M. Arian, A.R. Thierry, I.Pastan, M.G. Pang, W.G. Kearns, M.M Gottesman. Expression of the Human Multidrug Resistance and Glucocerebrosidase cDNAS from Adeno-Associated Vectors : Efficient Promoter Activity of AAV Sequences and in vivo Delivery via liposomes. Human. Gene Ther. 7 :1309-1322, July 10, 1996.
[24]  A.R. Thierry, P. Rabinovich, B. Peng, L. C. Mahan, J. L. Bryant, and R. C. Gallo. Characterization of liposome-mediated gene delivery : Expression, stability and pharmacokinetics of plasmid DNA. Gene Therapy 4:226-237, 1997.
[25]  C. Lavigne and A.R. Thierry. Enhanced antisense inhibition of human immunodeficiency virus type 1 in cell cultures by DLS delivery system. Biochem.Biophys.Res.Comm. 237 (3):566-571, 1997.
[26]  G. B. Takle, A. R. Thierry, S. M. Flynn, B. Peng, L. White, W. Devonhish, R. A. Galbraith, A. R. Goldberg, and S. T. George. Delivery of oligoribonucleotides to human hepatoma cells using cationic lipid particles conjugated to ferric protoporphyrin IX (heme). Antisense Nucl.Acid Drug Devlpt. 7:177-185, 1997.
[27]  A.R. Thierry. Optimization of lipoplex formulations for intravenous gene delivery. J.Liposome Res. 7:143-159, 1997.
[28]  Felgner, P.L., Barenholz, Y., Behr, J.P., Cheng, S.H., Cullis, P., Huang, L., Jessee, J.A., Seymour, L., Szoka, F., Thierry, A.R., Wagner, E., and Wu, G. Nomenclature for synthetic gene delivery systems. Hum. Gene Ther. 8, 511-512, 1997.
[29]  O. A. Sedelnikova, I. G. Panyutin, A. R. Thierry, and R. D. Neumann. Radiotoxicity of iodine-125-labeled oligodeoxyribonucleotides in mammalian cells. J.Nucl.Med. 39 (8):1412-1417, 1998.
[30]  F. Samaniego, J.L. Bryant, N. Liu, J.E. Karp, A .R. Thierry, Y. Lunardi-Iskandar, R.C. Gallo. Induction of program cell death in Kaposi's Sarcoma cells by preparations of human corionic gonadotropin. J. NCI. 91, 135-142, 1999.
[31]  M. Schmutz, D. Durand, Y, Palvadeau, A. Debin, A. Etienne, A.R. Thierry. DNA packing in stable lipid complexes designed for gene transfer imitates DNA compaction in bacteriophages. Proc. Nat. Acad. Sci. USA 96, 12293-12298, 1999.
[32]  C. Lavigne, J. Yelle, G. Sauvé, A.R. Thierry. Lipid-based delivery of combinations of antisense oligodeoxynucleotides for the in vitro inhibition of HIV-1 replication. AAPS Pharm. Sci. 3, article 7. 2001.
[33]  B. Tavitian, S. Marzabal, V. Boutet, B. Kuhnast, S. Terrazino, M. Moynier, F. Dollé, J.R. Deverre, A.R. Thierry. Characterization of a synthetic anionic vector for oligonucleotide Delivery using in vivo whole body dynamic imaging. Pharm. Res. 19, 367-376, 2002.
[34]  C. Lavigne, J. Yelle, G. Sauvé, A.R. Thierry. On the sequence specific activity of antisense oligonucleotides aimed at the inhibition of HIV-1 replication. AAPS Pharm. Sci., 4 article 9, (http ://www.pharmsci.org/) (2002).
[35]  A.R. Thierry, E. Vives, J.P. Richard, P. Prevot, C. Martinand-Mari, I. Robbins, B. Lebleu. Cellular Uptake and intracellular fate of antisense oligonucléotides. Current Op. Mol. Ther. 5, 133-138, (2003).
[36]  C. Lavigne, Y. Lunardi-Iskandar, B Lebleu, and A. R. Thierry. Cationic Liposomes/Lipids for Oligonucleotide Delivery: Application to the Inhibition of Tumorigenicity of Kaposi's Sarcoma by VEGF Antisense Oligodeoxynucleotides. Method Enzymol. 387, 189-210 (2004)
[37]  F.Maurel, F. Debart, F. Cavelier, A.R. Thierry, Lebleu Bernard, J.J Vasseur and E. Vivès. High yield synthesis of peptide-oligonucleotide chimera through a disulfide bridge: a simplified method for oligonucleotide activation. Bioconjugate Chem., in press; available online via ScienceDirect DOI information: 10.1016/j.bmcl.2005.07.086 (2005)
[38]  Abes, S., Williams, D., Prevot, A. Thierry, P., Gait, M. J., and Lebleu, B. Endosome trapping limits the efficiency of splicing correction by PNA-oligolysine conjugates, J Control Release 110(3):595-604 (2006)
[39]  A.R. Thierry, S. Abes, S. Resina, A. Travo, J.P. Richard, P. Prevot and B. Lebleu. Comparison of basic peptides-and lipid-based strategies for the delivery of splice correcting oligonucleotides. Biochim.Biophys.Acta, 1758(3):364-74 (2006)
[40]  A.R. Thierry, F. Kepes, P. Amar, G. Barlovatz, G. Bernot, C. Froidevaux, J.L. Giavitto, J. Guespin, V. Norris, V. Schafter, C. Godin and F. Molina. Modelling of biological complex systems in the context of genomics, J. Biol.Phys.Chem. 6, 103-107 (2006).
[41]  S. Resina, S. Abes, A. Travo, P. Clair, P. Prevot, R. Kole, B. Lebleu and A. R. Thierry. Switching on transgene expression by correcting aberrant splicing with using multi-targeting steric block oligonucleotides. J. Gene Med. 9, 498-510 (2007).
[42]  M. Meryet-Figuières, S. Resina, C. Lavigne, G. Barlovatz-Meimon, B. Lebleu and A.R. Thierry. Inhibition of PAI-1 Expression in Breast Cancer Carcinoma Cells by siRNA. Biochimie 89, 1228-1233 (2007)
[43]  Resina S., Abes S., Turner J.J., Prevot P., Travo A., Clair P., Gait M.J., Thierry A.R., Lebleu B. Lipoplex and peptide-based strategies for the delivery of steric-block oligonucleotides. Int J Pharm. 2007 May 17; [Epub ahead of print]
[44]  C. Lavigne and A.R. Thierry. Specific subcellular localization of siRNAs delivered by lipoplex in MCF-7 breast cancer cells. Biochimie, 89, 1245-1251 (2007)
[45]  Lavigne, C., Guignee, A., and Thierry, A. R. (2008) A rapid microwell fluorescence immunoassay for cellular protein detection. Biol Proced Online 10, 83-9.
[46]  Resina, S., Prevot, P., and Thierry, A. R. (2009) Physico-chemical characteristics of lipoplexes influence cell uptake mechanisms and transfection efficacy. PLoS One 4, e6058.
[47]  Thierry, A. R., Norris, V., Molina, F., and Schmutz, M. (2009) Lipoplex nanostructures reveal a general self-organization of nucleic acids. Biochim Biophys Acta 1790, 385-94.
[48]  Rialle, S., Felicori, L., Dias-Lopes, C., Pérès, S., El Atia, S., Thierry, A.R., Amar, P. and Molina, F. (2010). BioNetCAD: design, simulation and experimental validation of synthetic biochemical networks. Bioinformatics 26(18): 2298-304
[49]  Maltais M., Thierry A. R., Blessing T., Maicas E., Vert M. and Lavigne C. (2010) A biodegradable hydrogel as a cell delivery carrier for autologous tumor cell vaccines. Int.J.Oncol. 36, 617-622.
[50]  Thierry, A. R., Mouliere, F., Gongora, C., Ollier, J., Robert, B., Ychou, M., Del Rio, M., and Molina, F. (2010) Origin and quantification of circulating DNA in mice with human colorectal cancer xenografts. Nucleic Acids Res 38, 6159-75.
[51]  Arnau Peyrotte E., Mouliere, F., Gongora, C., Robert, B., Ychou, M., Del Rio, M., Molina, F and Thierry, A. R., , Study of the origin and quantification of circulating DNA in colorectal cancer mouse xenografts and patients: application to diagnosis, Bull. Cancer. 2010. 97: S48-49.
[52]  Norris, V., Zemirline, A., Amar, P., Audinot, J. N., Ballet, P., Ben-Jacob, E., Bernot, G., Beslon, G., Cabin, A., Fanchon, E., Giavitto, J. L., Glade, N., Greussay, P., Grondin, Y., Foster, J. A., Hutzler, G., Jost, J., Kepes, F., Michel, O., Molina, F., Signorini, J., Stano, P., and Thierry, A. R. (2010) Computing with bacterial constituents, cells and populations: from bioputing to bactoputing. Theory Biosci.
[53]  Lavigne, C, Slater, K, Duguay C, Arnau Peyrotte, E, Fortier G, Simard, M, Thierry AR Influence of lipoplex surface charge on siRNA delivery: Application to the in vitro downregulation of CXCR4 HIV-1 co-receptor, Molecular Pharmaceutics, accepted
[54]  Mouliere, F., Gongora, C., Robert, B., Pezet, D., Lamy, P.J., Crapez, E., Ychou, M., Del Rio, M., Molina, F and Thierry, A. R., , High fragmentation characterizes tumour-derived circulating DNA, PlosOne, accepted

Publications: Books/Book Chapters

[1]  Rahman, A., Dusre, L., Forst, D., Thierry, A., Roh, J.K and Treat, J. : Liposomal doxorubicin : preclinical and clinical data. In: Horizons in Membrane Technology-progress in Clinical and Biological Research. New York, Alan R. Liss (Ed.), 1990 pp 281-302
[2]  Thierry, A., Rahman, A., and Dritschilo, A. : Liposomal delivery as a new approach to transport oligonucleotides. In: Gene Regulation by Antisense RNA and DNA. Erickson, R.P. and Izant, J. (Eds), New York, Raven Press, 1992, pp 147-161
[3]  Thierry, A.R., and Tackle, G. : Liposomes as a delivery system for antisense and ribozyme compounds. In: Delivery Strategies for Antisense oligonucléotides. Akhtar, S. (Ed.)Boca Raton, CRC press, 1995.
[4]  Thierry, A.R., Circumventing transport-associated resistance through drug delivery. In: Drug Transport in Antimicrobial and Anticancer Chemotherapy. Georgopapadakou, N. (Ed.), New York, Marcel Dekker, Inc., p : 613-628, 1995.
[5]  A.R Thierry and L.C Mahan, Therapeutic Applications of lipid-based Gene Delivery systems, In: Advanced Gene Delivery, A. Rolland (Ed.), 123, 1998.
[6]  A .R. Thierry. Transfert de gènes à l'aide de vecteurs synthétiques. In : Actualités de Chimie Thérapeutique, In : Editions Scientifiques Médicales Elsevier, Paris, pp 101-103, 2001.
[7]  A. R. Thierry. Towards modelling and simulation of natural DNA compaction with a synthetic system. In: Modelling and simulation of biological processes in the context of genomics. Evry, pp167 (2004)
[8]  C. Godin, F. Molina and A.R. Thierry. Report of Conference synthesis In: Modelling of biological complex systems in the context of genomics. Genopole, P. Amar, F. Kepes, F. Molina and V. Norris, (Ed.), 2006.