American Journal of Biochemistry

American Journal of Biochemistry is a peer-reviewed international journal dedicated to provide an active forum to the global scientific community for publishing and disseminating high quality research work in the field of biochemistry.

Amritlal Mandal

Editorial Board Member of American Journal of Biochemistry

Post-doctoral Fellow, University of Arizona, USA

Research Areas

Cell Biology, Biochemistry, Physiology and Ophthalmology

Education

2005Ph.DBiochemistry, University of Kalyani, West Bengal, India
1998M.ScBiochemistry, University of Calcutta, Kolkata, India
1996B.ScChemistry Honors, University of Calcutta, Kolkata, India

Experience

2006-presentResearch Associate, AHSC, College of Medicine, Physiology
2006-2006Research Associate, University of Louisville, School of Medicine, Dept. of Ophthalmology and Visual Sciences
2000-2005Research Fellow, University of Kalyani, Faculty of Science, Dept. of Biochemistry and Biophysics
1998-2000Research Fellow, Indian Jute Industries' Research Association (IJIRA), Dept. of Biology

Academic Achievement

Expertise in Cell Biology, Molecular Biology, Protein purification, Microscopy, Imaging & transport biology
5+ years post-doctoral research experience (Research Associate, Department of Physiology, University of Arizona, Tucson, Arizona, USA, Aug 2006-till date) & (Research Associate, Department of Ophthalmology and Visual Sciences, University of Louisville, Lo
29 publications (22 research papers including 9 first authored, 6 review articles & 1 book chapter) in peer reviewed journals. One of the invited reviews (Mol. Cell. Biochem. 253, 269-285, 2003) has been cited 207 times so far and was most read and downlo
Talks presented in 7 Indian Institutes and Universities and in 2 US universities
Reviewed 3 articles in international journals
Supervisory experience with Ph.D. students and undergraduates
Posters presented in 18 international (including 5 ARVO and 2 Experimental Biology Annual meetings) and 3 national meetings
Actively participated in organizing 2 national seminars and 2 workshops at the University of Kalyani, Biochemistry and Biophysics department in the year 2002 and 2003

Membership

The American Physiological Society (APS), USA since 2008
The Association for Research in Vision and Ophthalmology (ARVO), USA since 2006
The Arizona Physiological Society (AZPS), USA since 2008

Publications: Conferences/Workshops/Symposiums/Journals/Books

[1]  A. Goldman, H.D.R. Chen, M.R. Khan, H. Rosely, K.A. Hill, M. Shahidullah, A. Mandal, N.A. Delamere and K. Dvorak. The Na+/H+ exchanger controls deoxycholic acid induced apoptosis by a H+-activated Na+-dependent ionic shift in esophageal cells. PLoS One (Research Article: In Press, Impact Factor: 4.351)
[2]  M. Shahidullah, A. Mandal, C. Beimgraben and N.A. Delamere. Hyposmotic stress causes ATP release and stimulates Na, K-ATPase activity in porcine lens. J Cell Physiol (May 26. doi: 10.1002/jcp.22858. [Epub ahead of print, PMID: 21618533] (Research Article, Impact Factor: 4.59)
[3]  A. Mandal, M. Shahidullah, C. Beimgraben and N.A. Delamere (2010). The effect of endothelin-1 on Src-family tyrosine kinases and Na, K-ATPase activity in porcine lens epithelium. J Cell Physiol (2010 Dec 28. [Epub ahead of print, PMID: 21190250) (Research Article, Impact Factor: 4.59)
[4]  K. Holthouser*, A. Mandal*, M.L. Merchant, J. Schelling, N.A. Delamere, R.R. Valdes Jr., S.C. Tyagi, E.D. Lederer, S.J. Khundmiri (2010). Ouabain stimulates Na, K-ATPase through sodium hydrogen exchanger-1 (NHE-1) dependent mechanism in human kidney proximal tubule cells. Am J Physiol Renal Physiol, 299 (1), F77-90 (Research Article, *Equal Contribution with K. Holthouser, Impact Factor: 4.199)
[5]  A. Mandal, M. Shahidullah, N.A. Delamere (2010). Hydrostatic pressure induced release of stored calcium in cultured rat optic nerve head astrocytes. Invest Ophthalmol Vis Sci, 51(6):3129-38 (Research Article, Impact Factor: 4.858)
[6]  M. Shahidullah, A. Mandal, N.A. Delamere (2009). Responses of sodium-hydrogen exchange to nitric oxide in porcine cultured nonpigmented ciliary epithelium, Invest Ophthalmol Vis Sci, 50(12):5851-8 (Research Article, Impact Factor: 4.858)
[7]  A. Mandal, M. Shahidullah, N. A. Delamere and M.A. Terán (2009) Elevated hydrostatic pressure activates sodium-hydrogen exchanger-1 in rat optic nerve head astrocytes, Am J Physiol Cell Physiol, 297(1):C111-20 (Research Article, Impact Factor: 4.334)
[8]  B. Ghosh, P. Kar, A. Mandal, K. Dey, T. Chakraborti, S Chakraborti (2009) Ca2+ influx mechanisms in caveolae vesicles of pulmonary smooth muscle plasma membrane under inhibition of α2β1 isozyme of Na+/K+-ATPase by ouabain, Life Sciences, 84(5-6)139-148 (Research Article, Impact Factor: 2.389)
[9]  A. Mandal, N.A. Delamere, M. Shahidullah (2008) Ouabain-induced stimulation of sodium-hydrogen exchange in rat optic nerve astrocytes, Am J Physiol Cell Physiol, 295(1), C110-11 (Research Article, Impact Factor: 4.334)
[10]  A. Mandal, S. Das, T. Chakraborti, P. Kar, B. Ghosh, S. Chakraborti (2006) Solubilization, purification and reconstitution of Ca2+-ATPase from bovine pulmonary artery smooth muscle microsomes by different detergents: Preservation of native structure and function of the enzyme by DHPC, Biochim Biophys Acta, 1760 (1), 20-31 (Research Article, Impact Factor: 2.958)
[11]  A. Mandal, T. Chakraborti, R. Choudhury, B. Ghosh, A.N. Ghosh, S. Das, and S. Chakraborti (2005) Role of MMP-2 in inhibiting Na+ dependent Ca2+ uptake by H2O2 in microsomes isolated from pulmonary smooth muscle, Mol Cell Biochem, 270 (1-2), 79-87 (Research Article, Impact Factor: 1.862)
[12]  A. Mandal, T.Chakraborti, R. Choudhury, B. Ghosh and S. Chakraborti (2005) Role of MMP-2 in oxidant-mediated regulation of Ca2+ uptake in microsomes of bovine pulmonary artery smooth muscle, Ind J Biochem. Biophys, 42(1), 19-27 (Research Article, 0.579)
[13]  S. Chakraborti, A. Mandal, S. Das and T. Chakraborti (2005) Role of MMP-2 in PKC delta-mediated inhibition of Na+ dependent Ca2+ uptake in microsomes of pulmonary smooth muscle: Involvement of a pertussis toxin sensitive protein, Mol Cell Biochem, 280 (1-2), 107-17 (Research Article, Impact Factor: 1.862)
[14]  S. Das, M. Mandal, T. Chakraborti, A. Mandal, S. Chakraborti (2004) Isolation of MMP-2 from MMP-2/TIMP-2 complex: characterization of the complex and the free enzyme in pulmonary vascular smooth muscle plasma membrane, Biochim Biophys Acta, 1674 (2), 158-174 (Research Article, Impact Factor: 2.958)
[15]  S. Das, M Mandal, A. Mandal, T. Chakraborti, S. Chakraborti (2004) Identification, purification and characterization of matrix metalloproteinase-2 in bovine pulmonary artery smooth muscle plasma membrane, Mol Cell Biochem, 258 (1-2), 73-89 (Research Article, Impact Factor: 1.862)
[16]  S Chakraborti, A. Mandal, S. Das and T. Chakraborti (2004) Inhibition of Na+/Ca2+ exchanger by peroxynitrite in microsomes of pulmonary smooth muscle: Role of matrix metalloproteinase-2, Biochim Biophys Acta, 1671 (1-3), 70-78 (Research Article, Impact Factor: 2.958)
[17]  A. Mandal, T. Chakraborti, S. Das, B. Ghosh, A.N. Ghosh and S. Chakraborti (2004) Matrix Metalloproteinase-2 Mediated Inhibition of Na+ Dependent Ca2+ Uptake by Superoxide Radical (O2-.) in Microsomes of Pulmonary Smooth Muscle, IUBMB Life, 56 (5), 267-276 (Research Article, Impact Factor: 3.578)
[18]  M. Mandal, S. Das, T. Chakraborti, A. Mandal and S. Chakraborti (2003) Role of the matrix metalloprotease-2 in the oxidant activation of Ca2+ATPase by hydrogen peroxide in pulmonary vascular smooth muscle plasma membrane, J Biosci, 28 (2), 205-213 (Research Article, Impact Factor: 1.703)
[19]  A. K Banerjee, A. Mandal, D. Chanda and S. Chakraborti (2003) Oxidant, antioxidant and Physical Exercise, Mol Cell Biochem, 253 (1-2), 307-312 (Review Article, Impact Factor: 1.862)
[20]  S. Chakraborti, M. Mandal, S. Das, A. Mandal and T.Chakraborti (2003) Regulation of matrix metalloproteinases: An overview, Mol Cell Biochem, 253 (1-2), 269-285 (Review Article, Impact Factor: 1.862)
[21]  S. Das, M. Mandal, T. Chakraborti, A. Mandal and S. Chakraborti (2003) Structure and evolutionary aspects of matrix metalloproteinases: A brief overview, Mol Cell Biochem, 253 (1-2), 31-40 (Review Article, Impact Factor: 1.862)
[22]  M. Mandal, A. Mandal, S. Das, T. Chakraborti, and S. Chakraborti (2003) Clinical Implications of Matrix metalloproteinases, Mol Cell Biochem, 252 (1-2), 305-329 (Research Article, Impact Factor: 1.862)
[23]  M. Mandal, S. Das, T. Chakraborti, A. Mandal and S. Chakraborti (2003) Identification, purification and partial characterization of tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in bovine pulmonary artery smooth muscle, Mol Cell Biochem, 254 (1-2), 145-155 (Research Article, Impact Factor: 1.862)
[24]  M. Mandal, A. Mandal, S. Das, T. Chakraborti and S. Chakraborti (2003) Identification, purification and partial characterization of tissue inhibitor of matrix metalloproteinase-2 in bovine pulmonary artery smooth muscle, Mol Cell Biochem, 254 (1-2), 275-287 (Research Article, Impact Factor: 1.862)
[25]  S. Das, T. Chakraborti, M. Mandal, A. Mandal and S. Chakraborti (2002) Role of a membrane associated Ca2+ dependent matrix metalloprotease in the activation of Ca2+-ATPase by tertiary butylhydroperoxide, Mol Cell Biochem, 237 (1-2), 85-93 (Research Article, Impact Factor: 1.862)
[26]  T. Chakraborti, M. Mandal, S.Das, A. Mandal, S. N. Ghosh and S. Chakraborti (2002) Protective Role of Magnesium in Cardiovascular Diseases: A Review, Mol Cell Biochem, 238 (1-2), 163-179 (Review Article, Impact Factor: 1.862)
[27]  T. Chakraborti, S. Das, M. Mandal, A. Mandal and S. Chakraborti (2002) Role of Ca2+ dependent matrix metalloprotease-2 in stimulating Ca2+ATPase activity under peroxynitrite treatment in bovine pulmonary artery smooth muscle membrane, IUBMB Life, 53 (3), 167-173 (Research Article, Impact Factor: 3.578)
[28]  T. Chakraborti, S. Das, M. Mandal, A. Mandal and S. Chakraborti (2002), Ca2+ dynamics under oxidant stress in the cardiovascular system, Molecular and cellular responses to stress, Vol 2, New York, Elsevier Science, 213-224 (Book Chapter: #16)
[29]  T. Chakraborti, A. Mandal, M. Mandal, S. Das and S. Chakraborti (2000) Complement activation in heart diseases: Role of oxidants, Cell Signal, 12 (9-10), 607-617 (Review Article, Impact Factor: 4.102)
[30]  Hyposmotic stress causes a cytoplasmic calcium increase in cultured lens epithelium by a mechanism involving P2-purinoceptors and Src family tyrosine kinase. A. mandal, M. Shahidullah and N.A. Delamere. ARVO 2011 Annual Meeting held on 1-5 May at Fort Lauderdale, FL, USA).
[31]  Hyposmotic stress causes ATP release and stimulates Na, K-ATPase activity in porcine lens. M. Shahidullah, A. Mandal, and N.A. Delamere. ARVO 2011 Annual Meeting held on 1-5 May at Fort Lauderdale, FL, USA).
[32]  AHSC Forntiers in Biomedical Research, October 7, 2009, held at the University of Arizona, Tucson, AZ USA. Title: The effect of endothelin-1 on Src-family tyrosine kinases and Na, K-ATPase activity in porcine lens epithelium. A. Mandal, M.S. Stepia, M. Shahidullah, C. Beimgraben and N.A. Delamere. Abstract # 40
[33]  Src family tyrosine kinase activation is required for endothelin-1 to inhibit Na, K-ATPase in porcine lens epithelium. N.A. Delamere, M. Shahidullah, C. Beimgraben and A. Mandal. Acta Ophthalmologica, Special Issue: Abstracts from the 2010 European Association for Vision and Eye Research Conference, Crete, Greece: Oct 6-9, 2010, Volume 88, Issue Supplement s246, September 2010.
[34]  Experimental Biology (APS) Annual Meeting, April 24-28, 2010 held at Anaheim, CA, USA. Title: Low dose ouabain regulation of Na, K-ATPase requires NHE-1 scaffolding properties. SJ Khundmiri, K Holthouser, A. Mandal, ML Merchant, J Schelling, NA Delamere and ED Lederer. Abstract # 798.5
[35]  Experimental Biology (APS) Annual Meeting, April 24-28, 2010 held at Anaheim, CA, USA. Title: Evidence for aldosterone-mediated regulation of Na, K-ATPase in kidney proximal tubules. J Parks, M Barati, SA sayler, A. Mandal, JD Klein and SJ Khundmiri. Abstract # 606.25
[36]  Presented at the ARVO Annual Meeting, May 2-6, 2010 held at Fort Lauderdale, FL, USA. Title: Endothelin-1 Inhibits Na, K-ATPase Activity Through a Src Family Kinase Mediated Mechanism in Porcine Lens Epithelium. A. Mandal, M. Shahidullah, C. Beimgraben, N.A. Delamere.
[37]  AZPS 2nd Annual Meeting, November 21-22, 2009, held at the University of Arizona, Tucson, AZ, USA. Title: Endothelin-1 inhibits Na, K-ATPAse through a Src Family Kinase (SFK) mediated mechanism in porcine lens epithelium. A. Mandal, M. Shahidullah, C. Beimgraben and N.A. Delamere.
[38]  AHSC Forntiers in Biomedical Research, October 7, 2009, held at the University of Arizona, Tucson, AZ USA. Title: Increased hydrostatic pressure causes release of stored calcium by cultured rat optic nerve head astrocytes. A. Mandal, M. Shahidullah and N.A. Delamere.
[39]  ARVO Annual Meeting, May 3-7, 2009 held at Fort Lauderdale, FL, USA. Title: Hydrostatic Pressure Causes Ryanodine Receptor-Mediated Calcium Release in Cultured Rat Optic Nerve Astrocytes. A. Mandal, M. Shahidullah and N.A. Delamere.
[40]  Experimental Biology (APS) Annual Meeting, April 18-22, 2009 held at New Orleans, LA, USA. Title: Elevated Hydrostatic Pressure Activates Sodium-Hydrogen Exchanger-1 in Rat Optic Nerve Head Astrocytes. A. Mandal, M. Shahidullah, N.A. Delamere and M.A Terán.
[41]  Experimental Biology (APS) Annual Meeting, April 18-22, 2009 held at New Orleans, LA, USA. Title: Low dose ouabain regulation of Na, K ATPase requires NHE-1 scaffolding properties. K. A. Holthouser, A. Mandal,, M. L. Merchant, J. Schelling, N.A. Delamere, R R. Valdes Jr., S.C. Tyagi, E. D. Lederer and S.J. Khundmiri.
[42]  AZPS Inaugural Meeting, November 7-8, 2008, held at the University of Arizona, Tucson, AZ, USA. Title: Pressure-induced stimulation of sodium-hydrogen exchanger 1 (NHE1) in cultured rat optic nerve astrocytes. A. Mandal, M. Shahidullah and N.A. Delamere.
[43]  43rd Annual Biological Transport Meeting (Affiliated with American Physiological Society) June 15-June 18, 2008 Lake Cumberland, Jamestown, KY, USA. Low dose ouabain increases Na, K-ATPase activity in rat kidney basolateral membranes. K. Holthouser, A. Mandal, N.A Delamere, E. Lederer and S.J. Khundmiri.
[44]  AHSC Forntiers in Biomedical Research, October 15, 2008, held at the University of Arizona, Tucson, AZ, USA. Title: Differential handling of pH swings by the porcine ocular nonpigmented ciliary epithelium. M Shahidullah, A. Mandal and N.A. Delamere.
[45]  ARVO Annual Meeting, April 27, 2008-May 1, 2008 held at Fort Lauderdale, FL, USA. Title: Ouabain stimulation of sodium-hydrogen exchange in rat optic nerve astrocytes involves calcium entry. A. Mandal, M. Shahidullah and N.A. Delamere.
[46]  Experimental Biology (APS) Annual Meeting, April 4-9, 2008 held at San Diego, CA, USA. Title: Inhibition of capacitative calcium entry suppresses ouabain-induced stimulation of sodium-hydrogen exchange in rat optic nerve astrocytes. A. Mandal, M. Shahidullah and N.A. Delamere.
[47]  ARVO Annual Meeting, May 6-10, 2007 held at Fort Lauderdale, FL, USA. Title: Effect of ouabain on pH recovery in cultured rat optic nerve astrocytes. A. Mandal, M. Shahidullah and N.A. Delamere.
[48]  National Seminar on "Recent Advances in Stress Physiology, Toxicology and Immunology", organized by University of Kalyani on November 27-28, 2003. Role of oxidants and sulfhydryl modifying agents on MMP-2 and MMP-2/TIMP-2 complex in pulmonary vascular smooth muscle membrane. S. Das, A. Mandal and S. Chakraborti.
[49]  National Seminar on "Recent Advances in Molecular Physiology", organized by University of Kalyani on 4-6 February, 2002. Role of a Ca2+ dependent matrix metalloprotease in stimulating Ca2+-ATPase activity under peroxynitrite treatment in bovine pulmonary artery smooth muscle membrne. M. Mandal, T. Chakraborti, S. Das, A. Mandal and S. Chakraborti.
[50]  National Seminar on "Recent Advances in Molecular Physiology", organized by University of Kalyani on 4-6 February, 2002. Role of Ca2+ dependent matrix metalloprotease-2 in the oxidant activation of Ca2+-ATPase by hydrogen peroxide in pulmonary smooth muscle membrane. S. Das, T. Chakraborti, M. Mandal, A. Mandal and S. Chakraborti.