Journal of Microbiology Research

Journal of Microbiology Research aims to publish all the latest and outstanding research articles, reviews and letters in all areas of major importance to techniques of microbiology and applied research. It publishes high quality research and review papers on novel aspects of microbiology, including environmental, food, agricultural, medical, pharmaceutical, veterinary, soil, water and biodeterioration.

Aran Incharoensakdi

Editorial Board Member of Journal of Microbiology Research

Professor, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Thailand

Research Areas

Mechanism Of Salinity Tolerance in Cyanobacteria,Biochemical Responses to Osmotic Stress in Cyanobacteria,Signal Transduction in Cyanobacteria Under Environmental Stress,Polyamines Metabolism in Cyanobacteria,Solutes Transport in Cyanobacteria,Biohydrogen

Education

2000-2002PostdoctoralMeijo University, Nagoya, Japan
1986Ph.DPlant Biochemistry, Nagoya University, Japan
1979M.ScBiochemistry, Mahidol University, Thailand
1976B.ScBiochemistry, Victoria University of Wellington, New Zealand

Experience

2006Professor, Department of Biochemistry, Faculty of Science, Chulalongkorn University
1990Associate Professor, Department of Biochemistry, Faculty of Science, Chulalongkorn University
1987Assistant Professor, Department of Biochemistry, Faculty of Science, Chulalongkorn University
2004-2008Head of Department of Biochemistry (CU)

Publications: Conferences/Workshops/Symposiums/Journals/Books

[1]  Baebprasert W, Jantaro S, Khetkorn W, Lindblad P, Incharoensakdi A. (2011) Increased H2 production in the cyanobacterium Synechocystis sp. strain PCC 6803 by redirecting the electron supply via genetic engineering of the nitrate assimilation pathway. Metab. Eng. 13: 610-616.
[2]  Baebprasert W, Karnchanatat A, Lindblad P, Incharoensakdi A. (2011) Na+-stimulated nitrate uptake with increased activity under osmotic upshift in Synechocystis sp. strain PCC 6803. World J. Microbiol. Biotechnol. (in press).
[3]  Yodsang P, Raksajit W, Brandt A-M, Salminen T.A, Mäenpää P, Incharoensakdi A. (2011) Recombinant polyamine-binding protein of Synechocystis sp. PCC 6803 specifically binds to and is induced by polyamines. Biochemistry (Moscow) 76: 713-719.
[4]  Soontharapirakkul K, Promden W, Yamada N, Incharoensakdi A, Iwamoto-Kihara A, Takabe T (2011) Halotolerant cyanobacterium Aphanothece halophytica contains a Na+-dependent F1F0-ATP synthase with potential role in salt tolerance. J. Biol. Chem. 286: 10169-10176.
[5]  Jantaro S, Pothipongsa A, Khanthasuwan S, Incharoensakdi A (2011) Short-term UV-B and UV-C radiations preferentially decrease spermidine contents and arginine decarboxylase transcript levels of Synechocystis sp. PCC 6803. Curr. Microbiol. 62: 420-426.
[6]  Maneeruttanarungroj C, Lindblad P, Incharoensakdi A (2010) A newly isolated green alga Tetraspora sp. CU2551 from Thailand with efficient hydrogen production. Int. J. Hydrogen Energy 35: 13193-13199.
[7]  Khetkorn W, Lindblad P, Incharoensakdi A (2010) Enhanced biohydrogen production by the N2-fixing cyanobacterium Anabaena siamensis strain TISTR 8012. Int. J. Hydrogen Energy 35: 12767-12776.
[8]  Agervald A, Baebprasert W, Zhang X, Incharoensakdi A, Lindblad P, Stensjö K (2010) The CyAbrB transcription factor CalA regulates the iron superoxide dismutase in Nostoc sp. strain PCC 7120. Environ. Microbiol. 12: 2826-2837.
[9]  Brandt AM, Raksajit W, Yodsang P, Mulo P, Incharoensakdi A, Salminen TA, Mäenpää P (2010) Characterization of the substrate-binding PotD subunit in Synechocystis sp. PCC 6803. Arch. Microbiol. 192: 791-801.
[10]  Soontharapirakkul K, Incharoensakdi A (2010) Na+-stimulated ATPase of alkaliphilic halotolerant cyanobacterium Aphanothece halophytica translocates Na+ into proteoliposomes via Na+ uniport mechanism. BMC Biochemistry 11: 30.
[11]  Baebprasert W, Lindblad P, Incharoensakdi A (2010) Response of H2 production and Hox-hydrogenase activity to external factors in the unicellular cyanobacterium Synechocystis sp. PCC 6803. Int. J. Hydrogen Energy. 35: 6611-6616.
[12]  Bualuang A, Soontharapirakkul K, Incharoensakdi A (2010) Na+/H+ exchange activity in the alkaliphile halotolerant cyanobacterium Aphanothece halophytica. J. Appl. Phycol. 22: 123-129.
[13]  Brandt AM, Raksajit W, Mulo P, Incharoensakdi A, Salminen TA, Mäenpää P (2009) Transcriptional regulation and structural modeling of the FutC subunit of an ABC-type iron transporter in Synechocystis sp. strain PCC 6803. Arch. Microbiol. 191: 561-570.
[14]  Raksajit W, Yodsang P, Mäenpää P, Incharoensakdi A (2009) Characterization of spermidine transport system in a cyanobacterium, Synechocystis sp. PCC 6803. J. Microbiol. Biotech. 19: 447-454.
[15]  Burut-Archanai S, Incharoensakdi A, Eaton-Rye JJ (2009) The extended N-terminal region of SphS is required for detection of external phosphate levels in Synechocystis sp. PCC 6803. Biochem. Biophys. Res. Commun. 378: 383-388.
[16]  Krungkrai J, Incharoensakdi A, Tungpradabkul S (2008) Biochemistry research in Thailand: Present status and forsight studies. ScienceAsia 34: 1-6.
[17]  Juntarajumnong W, Incharoensakdi A, Eaton-Rye JJ (2007) Identification of the start codon for SphS encoding the phosphate-sensing histidine kinase in Synechocystis sp. PCC 6803. Curr. Microbiol. 55: 142-146.
[18]  Juntarajumnong W, Eaton-Rye JJ, Incharoensakdi A (2007) Two-component signal transduction in Synechocystis sp. PCC 6803 under phosphate limitation: role of acetyl phosphate. J. Biochem. Mol. Biol. 40: 708-714.
[19]  Juntarajumnong W, Hirani TA, Simpson JM, Incharoensakdi A, Eaton-Rye JJ (2007) Phosphate sensing in Synechocystis sp. PCC 6803:SphU and the SphS-SphR two-component regulatory system. Arch. Microbiol. 188:389-402.
[20]  Wiangnon K, Raksajit W, Incharoensakdi A (2007) Presence of a Na+-stimulated P-type ATPase in the plasma membrane of the alkaliphilic halotolerant cyanobacterium Aphanothece halophytica. FEMS Microbiol. Lett. 270: 139-145.
[21]  Thaivanich S, Incharoensakdi A (2007) Purification and characterization of nitrate reductase from the halotolerant cyanobacterium Aphanothece halophytica. World J. Microbiol. Biotechnol. 23:85-92.
[22]  Phunpruch S, Baebprasert W, Thongpeng C, Incharoensakdi A (2006) Nucleotide sequencing and transcriptional analysis of uptake hydrogenase genes in the filamentous N2-fixing cyanobacterium Anabaena siamensis. J. Appl. Phycol. 18:713-722.
[23]  Laloknam S, Tanaka K, Buaboocha T, Waditee R, Incharoensakdi A, Hibino T, Tanaka Y, Takabe T (2006) Halotolerant cyanobacterium Aphanothece halophytica contains a betaine transporter active at alkaline pH and high salinity. Appl. Environ. Microbiol. 72:6018-6026.
[24]  Jantaro S, Kidron H, Chesnel D, Incharoensakdi A, Mulo P, Salminen T, Mäenpää P (2006) Structural modeling and environmental regulation of arginine decarboxylase in Synechocystis sp. PCC 6803. Arch. Microbiol. 184: 397-406.
[25]  Raksajit W, Mäenpää P, Incharoensakdi A (2006) Putrescine transport in a cyanobacterium Synechocystis sp. PCC 6803. J. Biochem. Mol. Biol. 39: 394-399.
[26]  Wutipraditkul N, Waditee R, Incharoensakdi A, Hibino T, Tanaka Y, Nakamura T, Shikata M, Takabe T, Takabe T (2005) Halotolerant cyanobacterium Aphanothece halophytica contains NapA-type Na+/H+ antiporters with novel ion specificity that are involved in salt tolerance at alkaline pH. Appl. Environ. Microbiol. 71:4176-4184.
[27]  Jantaro S, Mulo P, Jansen T, Incharoensakdi A, Mäenpää P (2005) Effects of long-term ionic and osmotic stress conditions on photosynthesis in the cyanobacterium Synechocystis sp. PCC 6803. Funct. Plant Biol. 32:807-815.
[28]  Incharoensakdi A, Laloknam S (2005) Nitrate uptake in the halotolerant cyanobacterium Aphanothece halophytica is energy-dependent driven by Delta pH. J. Biochem. Mol. Biol. 38:468-473.
[29]  Jantaro S, Mäenpää P, Mulo P, Incharoensakdi A (2003) Content and biosynthesis of polyamines in salt and osmotically stressed cells of Synechocystis sp. PCC 6803. FEMS Microbiol. Lett. 228:129-135.
[30]  Incharoensakdi A, Karnchanatat A (2003) Salt stress enhances choline uptake in the halotolerant cyanobacterium Aphanothece halophytica. Biochim. Biophys. Acta. 1621:102-109.
[31]  Incharoensakdi A, Wangsupa J (2003) Nitrate uptake by the halotolerant cyanobacterium Aphanothece halophytica grown under non-stress and salt-stress conditions. Curr. Microbiol. 47:255-259.
[32]  Inthorn D, Dungkokkrauad N, Incharoensakdi A (2003) Improved efficiency for the removal of cadmium from aqueous solution using cells of the cyanobacterium Phormidium angustissimum pre-treated with sodiumhydroxide. Asian J. Microbiol. Biotech. Env. Sci. 5:283-289.
[33]  Waditee R, Hibino T, Nakamura T, Incharoensakdi A, Takabe T (2002) Overexpression of a Na+/H+ antiporter confers salt tolerance on a freshwater cyanobacterium, making it capable of growth in sea water. Proc. Natl. Acad. Sci. USA. 99:4109-4114.
[34]  Waditee R, Hibino T, Tanaka Y, Nakamura T, Incharoensakdi A, Hayakawa S, Suzuki S, Futsuhara Y, Kawamitsu Y, Takabe T, Takabe T (2002) Functional characterization of betaine/proline transporters in betaine-accumulating mangrove. J. Biol. Chem. 277:18373-18382.
[35]  Incharoensakdi A, Kitjaharn P (2002) Zinc biosorption from aqueous solution by a halotolerant cyanobacterium Aphanothece halophytica. Curr. Microbiol. 45:261-264.
[36]  Incharoensakdi A, Hibino T, Takabe T (2002) Glu 103 Gln site-directed mutation causes an alteration in physical properties of spinach betaine aldehyde dehydrogenase. J. Biochem. Mol. Biol. Biophys. 6:243-248.
[37]  Inthorn D, Silapanuntakul S, Incharoensakdi A (2002) Filamentous cyanobacteria can efficiently remove cadmium present in aqueous solution at low concentration. Asian J. Microbiol. Biotech. Env. Sci. 4:1-6.
[38]  Inthorn D, Sidtitoon N, Silapanuntakul S, Incharoensakdi A (2002) Sorption of mercury, cadmium and lead by microalgae. ScienceAsia 28:253-261.
[39]  Waditee R, Hibino T, Tanaka Y, Nakamura T, Incharoensakdi A, Takabe T (2001) Halotolerant cyanobacterium Aphanothece halophytica contains an Na(+)/H(+) antiporter, homologous to eukaryotic ones, with novel ion specificity affected by C-terminal tail. J. Biol. Chem. 276:36931-36938.
[40]  Waditee R, Incharoensakdi A (2001) Purification and kinetic properties of betaine-homocysteine methyltransferase from Aphanothece halophytica. Curr. Microbiol. 43:107-111.
[41]  Inthorn D, Incharoensakdi A, Sidtitoon N (2001) Removal of mercury, cadmium and lead in aqueous solution by microalgae. Asian J. Microbiol. Biotech. Env. Sci. 3:109-115.
[42]  Inthorn D, Wongsirikul D, Ouijirakul K, Phoopat S, Incharoensakdi A (2001) Study on utilization of Spirulina platensis for treating wastewater from a soft-drink factory. Asian J. Microbiol. Biotech. Env. Sci. 3:101-107.
[43]  Incharoensakdi A, Matsuda N, Hibino T, Meng YL, Ishikawa H, Hara A, Funaguma T, Takabe T, Takabe T (2000) Overproduction of spinach betaine aldehyde dehydrogenase in Escherichia coli. Structural and functional properties of wild-type, mutants and E. coli enzymes. Eur. J. Biochem. 267:7015-7023.
[44]  Incharoensakdi A, Waditee R (2000) Degradation of glycinebetaine by betaine-homocysteine methyltransferase in Aphanothece halophytica: effect of salt downshock and starvation. Curr. Microbiol. 41:227-231.
[45]  Incharoensakdi A, Wutipraditkul N (1999) Accumulation of glycinebetaine and its synthesis from radioactive precursors under salt-stress in the cyanobacterium Aphanothece halophytica. J. Appl. Phycol. 11:515-523.
[46]  Incharoensakdi A, Wutipraditkul N, Kum-arb U (1999) Factors affecting the accumulation of glycine betaine in a halophilic cyanobacterium, Aphanothece halophytica. J. Sci. Res. Chula. Univ. 24:21-30.
[47]  Incharoensakdi A, Kum-arb U (1998) Betaine aldehyde dehydrogenase from a halotolerant cyanobacterium Aphanothece halophytica : Purification, properties, and regulation by salinity. J. Sci. Soc. Thailand. 24:231-240.
[48]  Incharoensakdi A, Kitjaharn P (1998) Removal of lead from aqueous solution by filamentous cyanobacterium, Spirulina platensis. J. Sci. Res. Chula. Univ. 23:37-44.
[49]  Kitjaharn P, Incharoensakdi A (1992) Factors affecting the accumulation of lead by Aphanothece halophytica. J. Sci. Res. Chula. Univ. 17:141-147.
[50]  Incharoensakdi A, Inthorn D (1992) Purification of phycocyanin from Spirulina isolated from Makkasan lagoon. In "Research in Photosynthesis". Murata N (ed.) Vol. III, 103-106. Kluwer, Dordrecht, The Netherlands.
[51]  Takabe T, Incharoensakdi A, Arakawa K, Yokota S (1988) CO2 fixation rate and RuBisCO content increase in the halotolerant cyanobacterium, Aphanothece halophytica, grown in high salinities. Plant Physiol. 88:1120-1124.
[52]  Incharoensakdi A, Takabe T (1988) Determination of intracellular chloride ion concentration in a halotolerant cyanobacterium Aphanothece halophytica. Plant Cell Physiol. 29:1073-1075.
[53]  Incharoensakdi A, Takabe T (1987) Structure, function, and regulation of ribulose-1, 5-bisphosphate carboxylase/oxygenase. J. Sci. Soc. Thailand 13:133-157.
[54]  Takabe T, Incharoensakdi A (1986) Function and gene expression of RuBisCO. J. Jap. Biochem. Soc. 58: 175-182.
[55]  Incharoensakdi A, Takabe T, Akazawa T (1986) Effect of betaine on enzyme activity and subunit interaction of ribulose-1,5-bisphosphate carboxylase/oxygenase from Aphanothece halophytica. Plant Physiol. 81:1044-1049.
[56]  Incharoensakdi A, Takabe T, Akazawa T (1986) Role of the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase in the activation process. Arch. Biochem. Biophys. 248:62-70.
[57]  Incharoensakdi A, Takabe T, Takabe T, Akazawa T (1986) Isolation of the catalytically competent small subunit of ribulose bisphosphate carboxylase/oxygenase from spinash under an extremely alkaline condition. Biochem. Biophys. Res. Commun. 138:118-124.
[58]  Incharoensakdi A, Takabe T, Akazawa T (1985) Factors affecting the dissociation and reconstitution of ribulose-1,5-bisphosphate carboxylase/oxygenase from Aphanothece halophytica. Arch. Biochem. Biophys. 237:445-453.
[59]  Incharoensakdi A, Takabe T, Takabe T, Akazawa T (1985) Heterologous hybridization of ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) restores the enzyme activities. Biochem. Biophys. Res. Commun. 126:698-704.
[60]  Takabe T, Incharoensakdi A, Akazawa T (1984) Essentiality of the small subunit (B) in the catalysis of RuBP carboxylase/oxygenase is not related to substrate-binding in the large subunit (A). Biochem. Biophys. Res. Commun. 122:763-769.
[61]  Incharoensakdi A, Panyim S (1981) In vitro decondensation of human sperm chromatin. Andrologia 13:64-73.