International Journal of Biological Engineering

International Journal of Biological Engineering is an international peer-reviewed multidisciplinary journal publishing original scientific research in all fields of biological pharmacy. Original research work that contributes significantly to further the scientific knowledge in biological pharmacy will be appreciated.

Margaret Tzaphlidou

Editorial Board Member of International Journal of Biological Engineering

Professor, Medical Physics Department, Medical School, Ioannina University, Greece

Research Areas

Health Sciences, Biophysics, Biomedical Engineering, Electromagnetism, Spectroscopy

Education

1977-1980Ph.DMedical Biophysics, Department of Medical Biophysics, Medical School, University of Manchester, U.K.
1971-1975B.ScUniversity of Ioannina, Department of Physics

Experience

2011-presentDean of Medical School, University of Ioannina
2009-2011Vice Dean of Medical School, University of Ioannina
2006-2009 Director of Division of Clinical & Basic Morphological Sciences, Medical School, University of Ioannina, Greece

Academic Achievement

1978-1980: Alexandros Onasis Organization PhD fellowship (Manchester University, UK)
1981,1983,1984: British Council fellowships to carry on postdoc research in Medical Biophysics Department, Manchester University, UK

Publications: Journals

[1]  M. Tzaphlidou, J.A. Chapman and K.M. Meek. A study of positive staining for electron microscopy using collagen as a model system. I. Staining by phosphotungstate and tungstate ions. Micron, 13, 119-131, 1982.
[2]  M. Tzaphlidou, J.A. Chapman and M.H. Al-Samman. A study of positive staining for electron microscopy using collagen as a model system. II. Staining by uranyl ions. Micron, 13, 133-145, 1982
[3]  M. Tzaphlidou. The effects of fixation by combination of glutaraldehyde/dimethyl suberimidate. Use of collagen as a model system. J. Histoch. Cytoch. 31, 1274-1278, 1983.
[4]  M. Tzaphlidou and R.A. Hardcastle. A computer method for the comparison or the quantitative similarity between normal and abnormal collagen or between sequence data and experimental data. Int. J. Bio-Medical Computing, 15, 13-120, 1984
[5]  M. Tzaphlidou and J.A. Chapman. A study of positive staining for electron microscopy using collagen as a model system. III. The effects of suberimidate fixation. Micron Microscopica Acta, 15, 69-76, 1984
[6]  M. Tzaphlidou. Negative staining electron microscopy of collagen fibrils. "Invited Article" Micron Microscopica Acta, 7, 201-213, 1986
[7]  M. Tzaphlidou and J.A. Chapman. A study of staining for electron microscopy using collagen as a model system. IV. Phosphotungstate/tungstate negative staining patterns and their correlation with sequence data. Micron Microscopica Acta, 17, 269-280, 1986
[8]  M. Tzaphlidou. A study of staining for electron microscopy using collagen as a model system. V. The effect of suberimidate fixation on negative staining patterns. Micron Microscopica Acta, 17, 281-287, 1986
[9]  M. Tzaphlidou. Collagen cross-linking produced by dimethyladipimidate. Its comparison with dimethylsuberimidate. Micron Microscopica Acta, 18, 55-58, 1987
[10]  M. Tzaphlidou and J.A. Chapman. A study of staining for electron microscopy using collagen as a model system. VI. Uranyl nitrate negative staining patterns and their correlation with sequence data. Micron Microscopica Acta, 18, 147-151, 1987
[11]  D.P. Matthopoulos and M. Tzaphlidou. Tissue culture fixation with diimidoesters. I. Comparison of structure obtained with diimidoesters and formaldehyde. Micron Microscopica Acta, 18, 273-279, 1987
[12]  M. Tzaphlidou and D.P. Matthopoulos. Diimidoesters: Role in electron and light microscopy. "Invited Article" Micron Microscopica Acta, 19, 1-12, 1988
[13]  M. Tzaphlidou. Staining for electron microscopy by vanadyl sulfate. Use of collagen as a model system. Micron Micoscopica Acta, 19, 141-145, 1988
[14]  M. Tzaphlidou and D.P. Matthopoulos. Penetration rate of dimethyladipimidate. Use of gelatin gels as model system. Micron Microscopica Acta, 19, 137-139, 1988
[15]  D.P. Matthopoulos and M. Tzaphlidou. Tissue culture fixation with diimidoesters. II. The development of the vimentin type filament network of monkey kidney CV1 cells. Micron Microscopica Acta, 19, 13-17, 1988
[16]  D.P. Matthopoulos and M. Tzaphlidou. The effect of elevated temperature on the aggregation of African green monkey kidney cells. Cell Biol. Int. Rep., 12, 413-424, 1988
[17]  J.A. Chapman and M. Tzaphlidou. A study of staining for electron microscopy using collagen as a model system. VIII. Simulation of the negative staining patterns. Micron Microscopica Acta, 20, 75-87, 1989
[18]  D.P. Matthopoulos and M. Tzaphlidou. Tissue culture fixation with diimidoesters. III. Influence of adipimidate on cell size, thermal and mechanical stability. Micron Microscopica Acta, 20, 239-245, 1989
[19]  J.A. Chapman, M. Tzaphlidou, K.M. Meek and K.E. Kadler. The collagen fibril-A model system for studying the staining and fixation of a protein. Electron Microscopy Reviews, 3, 143-182, 1990
[20]  D.P. Matthopoulos and M. Tzaphlidou. Tissue culture fixation with diimidoesters. IV. A comparison between adipimidate and aldehydes of their effects on cell size and morphology. Micron Microscopica Acta, 21, 69-76, 1990
[21]  M. Tzaphlidou. The negative staining pattern of collagen fibrils as studied by electron microscopy. The effect of fixation. Micron Microscopica Acta, 22, 371-382, 1991
[22]  M. Tzaphlidou, K.C. Siamopoulos and D. Glaros. Abnormal collagen fibril structure of skin in chronic haemodialysis patients: An electron microscopic study. Micron Microscopica Acta, 22, 1-8, 1991
[23]  M. Tzaphlidou. The positive staining pattern of skin collagen in chronic haemodialysis patients. Micron Microscopica Acta, 22, 17-19, 1991
[24]  M. Tzaphlidou, I. Leontiou and D. Glaros. The effect of gamma irradiation on the organization of mouse skin collagen fibrils. The positive staining pattern. Micron MIcroscopica Acta, 22, 207-212, 1991
[25]  M. Tzaphlidou, C. Cummings and J.A. Chapman. A study of staining for electron microscopy using collagen as a model system. IX. The effect of tannic acid fixation. Micron Microscopica Acta, 23, 25-35, 1992
[26]  M. Tzaphlidou. Abnormal collagen fibril structure as studied by electron microscopy. Electron Microsc. Rev., 5, 25-35, 1992
[27]  I. Leontiou, D.P. Matthopoulos, M. Tzaphlidou and D. Glaros. The effect of gamma irradiation in collagen fibril structure. Micron, 24, 13-16, 1993.
[28]  E. Kounadi, M. Tzaphlidou and D. Glaros. An electron microscopic study of collagen fibril structure after lithium treatment-I. The effect of different lithium doses on mouse skin collagen. Micron, 25, 233-240, 1994.
[29]  E. Kounadi, M. Tzaphlidou, G. Fountos and D. Glaros. An electron microscopic study of collagen fibril structure after lithium treatment-II. The effects of low lithium dose and short treatment on mouse skin collagen. Micron, 26, 113-120, 1995.
[30]  D.P. Matthopoulos, M. Tzaphlidou and I. Leontiou. Morphological alterations caused by lithium in various cell lines. Cell Biol. Int., 19, 499-506, 1995.
[31]  M. Tzaphlidou, E. Kounadi, I. Leontiou, D.P. Matthopoulos and D. Glaros. The influence of low doses gamma irradiation on mouse skin collagen fibrils. International Journal of Radiation Biology, 71, 109-115,1997.
[32]  E. Kounadi, G. Fountos and M. Tzaphlidou. The influence of inflammation-mediated osteopenia (IMO) on the structure of rabbit bone and skin collagen fibrils. Connective Tissue Research, 37, 69-76, 1998.
[33]  M. Tzaphlidou and E. Kounadi. The effect of lithium treatment on collagenous tissues: an electron microscope study. Micron, 29, 235-248, 1998.
[34]  G. Fountos, E. Kounadi, M. Tzaphlidou, S. Yasumura and D. Glaros. The effects of inflammation-mediated osteoporosis (IMO) on the skeletal Ca/P ratio and on the structure of rabbit bone and skin collagen. Appl. Radiat. Isot., 49, 657-659, 1998.
[35]  G. Fountos, M. Tzaphlidou, E. Kounadi and D. Glaros. In vivo measurement of radius calcium/phosphorus ratio by X-ray absorptiometry. Appl. Radiat. Isot., 51, 273-278, 1999.
[36]  H. Kafantari, E. Kounadi, M. Fatouros, M. Milonakis and M. Tzaphlidou. Structural alterations in rat skin and bone collagen fibrils induced by ovariectomy. Bone, 26, 349-353, 2000.
[37]  M. Tzaphlidou and H. Kafantari. Influence of nutritional factors on bone collagen fibrils in ovariectomized rats. Bone, 27, 635-638, 2000.
[38]  M. Tzaphlidou, G. Fountos and D. Glaros. Bone Hydroxyapatite/Collagen Ratio: In Vivo Measurements by X-ray Absorptiometry. Annals of The New York Academy of Sciences, 904, 284-286, 2000.
[39]  M. Tzaphlidou, E. Kounadi and H. Kafantari. Influence of lithium on mouse bone collagen fibrils. J. Trace and Microprobe Techniques, 18, 321-326, 2000.
[40]  M. Tzaphlidou. Diameter distributions of collagenous tissues in relation to sex. A quantitative ultrastructural study. Micron, 32, 333-336, 2001.
[41]  M. Tzaphlidou. Measurement of the axial periodicity of collagen fibrils using an image processing method. Micron, 32, 337-339, 2001.
[42]  B. Eyden and M. Tzaphlidou. Structural variations of collagen in normal and pathological tissues: role of electron microscopy. Micron, 32, 287-300, 2001.
[43]  M. Tzaphlidou. Collagen as a model for the study of radiation induced side effects. Use of image processing. Micron, 33, 117-120, 2002.
[44]  M. Tzaphlidou and P. Berillis. Structural alterations caused by lithium in skin and liver collagen using an image processing method. J. Trace Microprobe Tech., 20, 493-504, 2002
[45]  V. Zaichick and M. Tzaphlidou. Determination of calcium, phosphorus, and the calcium/phosphorus ratio in cortical bone from the human femoral neck by neutron activation analysis. Applied Rad Isot., 56, 781-786, 2002.
[46]  M. Tzaphlidou and V. Zaichick. Neutron activation analysis of calcium/phosphorus ratio in rib bone of healthy humans. Applied Rad Isot., 57, 779-783, 2002.
[47]  M. Tzaphlidou. Side effects of lithium on rat cranial arachnoid and dura mater collagen. A quantitative ultrastructural study. J Trace Elem. Exp. Med., 16, 17-26, 2003.
[48]  V. Zaichick and M. Tzaphlidou. Calcium, phosphorus concentrations and the calcium/phosphorus ratio in trabecular bone from the femoral neck of healthy humans as determined by neutron activation analysis. Appl. Rad. Isotop., 58, 623-627, 2003.
[49]  M. Tzaphlidou and V. Zaichick. Calcium, Phosphorus, Calcium-Phosphorus ratio in rib bone of healthy humans. Biol. Trace Elem. Res., 93, 63-74, 2003.
[50]  M. Tzaphlidou and V. Zaichick. Sex and age related Ca/P ratio in cortical bone of iliac crest of healthy humans. J. Radioanal. Nucl. Chem., 259, 347-349, 2004.
[51]  M. Tzaphlidou. The role of collagen and elastin in aged skin: an image processing approach. Micron, 35, 171-175, 2004.
[52]  M. Tzaphlidou and M. Zervakis. Aged skin: detection of alterations of major collagen types ratio by image processing of electron-optical data. Micron, 35, 219-223, 2004.
[53]  M. Tzaphlidou and P. Berillis. Effect of lithium administration on collagen and breaking pressure of the rat thoracic descending aorta. J. Trace Elem. Exp. Med., 17, 151-160, 2004.
[54]  M. Tzaphlidou. Collagenous tissues upon lithium treatment: A quantitative ultrastructural study. TheScientificWorldJOURNAL 4, 605-621, 2004. URL: http://www.thescientificworldJOURNAL.com
[55]  M. Tzaphlidou and E. Fotiou. The effects of 910-MHz electromagnetic field on rat cranial arachnoid and dura mater collagen. The axial periodicity of collagen fibrils. TheScientificWorldJOURNAL 4 (S2),70-74, 2004. URL: http://www.thescientificworldJOURNAL.com
[56]  M. Tzaphlidou, E. Fotiou, Ch. Gousias and D.P. Matthopoulos. Development of a reliable and low cost system for the study of EMF biological effects. TheScientificWorldJOURNAL 4 (S2), 100-104, 2004. URL: http://www.thescientificworldJOURNAL.com
[57]  V. Kyriazis and M. Tzaphlidou. Skeletal calcium/phosphorus ratio measuring techniques and results, part I: microscopy and microtomography. TheScientificWorldJOURNAL 4, 1027-1034, 2004. URL: http://www.thescientificworldJOURNAL.com
[58]  M. Tzaphlidou, R. Speller, G. Royle, J. Griffiths, A. Olivo, S. Pani and R. Longo. High resolution Ca/P maps of bone architecture in 3-D synchrotron radiation microtomographic images. Appl. Rad. Isotop., 62, 569-575, 2005.
[59]  R. Speller, S. Pani, M. Tzaphlidou and J. Horrocks. MicroCT analysis of calcium/phosphorus ratio maps at different bone sites. Nuclear Instruments and Methods in Physics Research A, 548, 269-273, 2005.
[60]  A. Iglic, M. Tzaphlidou, M. Remskar, B. Babnik, M. Daniel and V. Kralj-Iglic. Stable shapes of thin anisotropic nano-strips. Fullerenes, Nanotubes and Carbon Nanostructures, 13, 183-192, 2005.
[61]  V. Kyriazis and M.Tzaphlidou. Skeletal calcium/phosphorus ratio measuring techniques and results: neutron activation analysis and x-ray absorptiometry. J. Radioanal. Nucl. Chem., 265, 519-525, 2005.
[62]  M. Zervakis, V. Gkoumplias and M. Tzaphlidou. Analysis of fibrous proteins from electron microscopy images. Medical Engineering and Physics, 27, 655-667, 2005.
[63]  V. Gkoumplias, M. Zervakis and M. Tzaphlidou. An automated approach for analyzing D-periods in collagen fibril images. IEEE Transactions on Biomedical Engineering, 52, 1345-1347, 2005.
[64]  M. Tzaphlidou. The role of collagen in bone structure: an image processing approach. Micron, 36, 593-601, 2005.
[65]  M. Tzaphlidou and P. Berillis. Collagen fibril diameter in relation to bone site. A quantitative ultrastructural study. Micron, 36, 703-705, 2005.
[66]  M. Tzaphlidou, P. Berillis and D. Matthopoulos. Bone calcium, phosphorus detection by Auger electron spectroscopy. Micron, 36, 706-709, 2005.
[67]  M. Tzaphlidou, R. Speller, G. Royle and J. Griffiths. Preliminary estimates of the calcium/phosphorus ratio at different cortical bone sites using synchrotron microCT. Physics in Medicine and Biology, 51, 1849-1855, 2006.
[68]  P. Berillis, D. Emfietzoglou and M. Tzaphlidou. Collagen fibril diameter in relation to bone site and to calcium/phosphorus ratio. TheScientificWorldJOURNAL, 6, 1109-1113, 2006. URL: http://www.thescientificworldJOURNAL.com
[69]  M. Tzaphlidou. Bone architecture: Collagen structure and calcium/phosphorus maps. Journal of Biological Physics, 34, 39-49, 2008.
[70]  N. Kourkoumelis, and M. Tzaphlidou. Spectroscopic assessment of normal cortical bone: differences in relation to bone site and sex. TheScientificWorldJOURNAL,10, 402-412, 2010. URL: http://www.thescientificworldJOURNAL.com
[71]  N. Kourkoumelis, and M. Tzaphlidou. Medical safety issues concerning the use of incoherent infrared light in biometrics. Lecture Notes in Computer Science, Springer-Verlag Berlin Heidelberg, 6005, 121-126, 2010.
[72]  N. Kourkoumelis, and M. Tzaphlidou. Multivariate statistical evaluation of bone site and sex as parameters for the Fourier transform infrared spectroscopic study of normal bone. Spectroscopy, 24, 99-104, 2010.
[73]  I. Balatsoukas, N. Kourkoumelis and M. Tzaphlidou. Auger electron spectroscopy for the determination of sex and age related Ca/P ratio at different bone sites. Journal of Applied Physics, 108, 074701-074701-6, 2010.
[74]  N. Kourkoumelis and M. Tzaphlidou. Eye safety related to near infrared radiation exposure to biometric devices. TheScientificWorldJOURNAL,11, 520-528, 2011. URL: http://www.thescientificworldJOURNAL.com
[75]  L. Astrakas, C. Gousias and M. Tzaphlidou. Electric field effects on chignolin conformation. Journal of Applied Physics, 109, 094702-1-094702-5, 2011.

Publications: Books/Book Chapters

[1]  M. Tzaphlidou. Role of the collagen fibril positive staining pattern in determining lithium side effects on collagen. In: 'Lithium-50 years: Recent advances in biology and medicine', Lucas, K.C., Becker, R.W., Gallicchio, V.S. eds. Weidner Publishing Group, Chesire, Connecticut, pp. 75-80, 1999.
[2]  M. Tzaphlidou. Role of cross-linking in collagen structural alterations brought about by lithium. In: 'Lithium-50 years: Recent advances in biology and medicine', Lucas, K.C., Becker, R.W., Gallicchio V.S. eds. Weidner Publishing Group, Chesire, Connecticut, pp. 117-122, 1999.
[3]  M. Tzaphlidou. Computer-aided Techniques for the Ultrastructural Study of a Protein. In: 'Modern Problems of Cellular and Molecular Biophysics', Ayrapetyan, S.N., North, A.C.T. eds. Noyan Tapan-Armenia by UNESCO Chair-Life Sciences International Educational Center, pp. 325-331, 2001.
[4]  M. Tzaphlidou. The Collagen Fibril-A Model System for Studying Structural Properties, as well as Staining and Fixation of a Protein. In: 'Modern Problems of Cellular and Molecular Biophysics', Ayrapetyan, S.N., North, A.C.T. eds. Noyan Tapan-Armenia by UNESCO Chair-Life Sciences International Educational Center, pp. 333-342, 2001.
[5]  M. Tzaphlidou. Side effects of lithium on various tissues: Use of collagen as a model system. In: 'Trends in Physiological Sciences. Cells to Systems', Mohammad Fahim ed., Serials Publications, Delhi-India, pp. 1-16, 2002.
[6]  M. Tzaphlidou and E. Fotiou. Collagen as a target for electromagnetic fields. Effects of 910-MHz on rat brain. In: 'BIOELECTROMAGNETICS Current Concepts'NATO Series-B: Physics and Biophysics, S.N. Ayrapetyan and M.S. Markov (eds.), Springer, The Netherlands, pp. 183-193, 2006.