American Journal of Biochemistry

American Journal of Biochemistry is a peer-reviewed international journal dedicated to provide an active forum to the global scientific community for publishing and disseminating high quality research work in the field of biochemistry.

Jose Renato Pinto

Editorial Board Member of American Journal of Biochemistry

Professor, Department of Biomedical Science, College of Medicine, Florida State University, USA

Research Areas

Cardiac and Skeletal Muscle Contraction and Regulation.Major Areas Include Biochemistry and Pharmacology

Education

2002-2006Ph.DMedical Biochemistry, Federal University of Rio de Janeiro-Brazil. Advisor: Martha M. Sorenson
2001-2002M.ScMedical Biochemistry, Federal University of Rio de Janeiro-Brazil

Experience

2010-present2010-present Research Assistant Professor, Molecular and Cellular Pharmacology, Miller School of Medicine, University of Miami – USA
2006-20102006-2010 Postdoctoral Associate, Molecular and Cellular Pharmacology, Miller School of Medicine, University of Miami – USA Mentor: James D. Potter, Ph.D
2001-2006Professor of Biochemistry, Physiology and Pharmacology, Faculdades Sao Jose, Rio de Janeiro, Brazil
2001-2006Guest Lecturer, Exercise Biochemistry (Liver and Muscle Metabolism, Mechanism of Fatigue, Training Adaptations and Mechanism of Muscle Contraction) Federal University of Rio de Janeiro, Brazil
2003Graduate Teaching Assistant, Pharmaceutical Biochemistry (Digestive System). Federal University of Rio de Janeiro, Brazil
2002Graduate Teaching Assistant, General Biochemistry (Bioenergetics). Federal University of Rio de Janeiro, Brazil
2001Graduate Teaching Assistant, General Biochemistry (Proteins and Enzymes). Federal University of Rio de Janeiro, Brazil

Academic Achievement

7/2008-6/2010 Postdoctoral Fellow, American Heart Association
Feb/2008 Travel Award Medical Faculty Association Margaret Whelan Fund, Miller School of Medicine University of Miami.
Feb/2006 International Travel Grant, to attend the 50th Biophysical Society Meeting, Salt Lake City, Feb. 2006
May/2002 Grant IUPAB/UNESCO, to attend the 14th International Biophysical Congress, Buenos Aires, May 2002
2002-2006 Graduate (Ph.D.) study fellowship (CNPq)
2001-2002 Graduate (M.Sc.) study fellowship (CAPES)
1997-2001 Undergraduate research fellowship (CNPq)

Membership

Member of the Brazilian Biochemistry and Molecular Biology Society (SBBq), Brazil
Member of the Biophysical Society, USA
Member of the American Heart Association, USA

Publications: Conferences/Workshops/Symposiums/Journals/Books

[1]  Midde K, Dumka V, Pinto JR, Muthu P, Marandos P, Gryczynski I, Gryczynski Z, Potter JD and Borejdo J. Myosin cross-bridges do not form precise rigor bonds in hypertrophic heart muscle carrying troponin T mutations. J Mol Cell Cardiol. 2011 Jun 12 (In Press)
[2]  Pinto JR, Yang SW, Hitz MP, Parvatiyar MS, Jones MA, Liang J, Kokta V, Talajic M, Tremblay N, Jaeggi M, Andelfinger G and Potter JD. Fetal Cardiac Troponin Isoforms Rescue the Increased Ca2+ Sensitivity Produced by a Novel Double Deletion in Cardiac Troponin T Linked to Restrictive Cardiomyopathy: A CLINICAL, GENETIC, AND FUNCTIONAL APPROACH. J Biol Chem. 2011 Jun 10;286(23):20901-12.
[3]  Pinto, J.R., Sousa, V.P. and Sorenson, M.M. Redox State of Troponin C Cysteine in the D/E Helix Alters the C-domain Affinity for the Thin Filament of Vertebrate Striated Muscle. BBA-Gen Subj. 1810: 391-397, 2011
[4]  Pinto, J.R., Reynaldo, D.P., Parvatiyar, M.S., Dweck, D., Liang, J., Jones, M.A., Sorenson, M.M. and Potter, J.D. Strong Crossbridges Potentiate the Ca2+ Affinity Changes Produced by HCM-Cardiac Troponin C Mutants in Myofilaments. A Fast Kinetic Approach. J. Biol. Chem. 286(2): 1005-1013, 2011
[5]  Morales, A., Pinto, J.R., Siegfried, J.D., Li, D., Norton, N., Hofmeyer, M., Vallin, M., Morales, A.R., Potter, J.D. and Hershberger, R.E. Late onset sporadic dilated cardiomyopathy caused by a cardiac troponin T mutation. Clin. Trans. Sci. 3:219-226, 2010
[6]  Parvatiyar, M.S., Pinto, J.R., Liang, J.S. and Potter, J.D. Predicting Cardiomyopathic Phenotypes by Altering the Ca2+ Affinity of Cardiac Troponin C. J. Biol. Chem. 285(36): 27785-97, 2010
[7]  Li, Y., Charles, P.W.J., Nan, C., Pinto, J.R., Wang, Y., Liang, J., Wu, G., Tian, J., Feng, H.Z., Potter, J.D., Jin, J.P. and Huang, X. Correcting diastolic dysfunction by desensitizing troponin in transgenic RCM mice. J. Mol. Cell. Card. 49: 402-411, 2010
[8]  Dweck, D., Reynaldo, D.P., Pinto, J.R. and Potter, J.D. A Dilated Cardiomyopathy Troponin C Mutation Lowers Contractile Force by Reducing Strong Myosin-Actin Binding. J. Biol. Chem. 285(23): 17371-17379, 2010
[9]  Parvatiyar, M.S.*, Pinto, J.R.*, Dweck, D. and Potter, J.D. Cardiac Troponin Mutations and Restrictive Cardiomyopathy. J. Biomed. Biotechnol. ID 350706, 2010 (*Co-first author).
[10]  Willott, R.H., Gomes, A.V., Parvatiyar, M.S., Chang, A., Pinto, J.R. and Potter, J.D. Mutation in Troponin that Cause Hypertrophic, Dilated and Restrictive Cardiomyopathy: What Can We Learn About Thin Filament Function? J. Mol. Cell Card. 48: 882-892, 2010
[11]  Wen, Y., Xu, Y., Wang, Y., Pinto, J.R., Potter, J.D. and Kerrick, W.G.L. Functional Effects of a Restrictive Cardiomyopathy linked Cardiac Troponin I mutation (R145W) in Transgenic Mice. J. Mol. Biol., 392(5): 1158-1167, 2009
[12]  Pinto, J.R., Parvatiyar, M.S., Jones, M.A., Liang, J., Ackerman, M.J. and Potter, J.D. A functional and structural study of troponin C mutations related to hypertrophic cardiomyopathy. J. Biol. Chem, 284(28): 19090-19100, 2009
[13]  Hershberger, R.E., Pinto, J.R., Parks, S.B., Kushner, J.D., Li, D., Ludwigsen, S., Cowan, J., Morales, A., Parvatiyar, M.S. and Potter, J.D. Clinical and functional characterization of TNNT2 mutations identified in patients with dilated cardiomyopathy. Circulation: Cardiovascular Genetics, 2: 306-313, 2009
[14]  Baudenbacher, F., Schober, T., Pinto, J.R., Sidorov, V., Hilliard, F., Solaro, R.J., Potter, J.D. and Knollmann, B.C. Myofilament Calcium Sensitization Causes Susceptibility to Cardiac Arrhythmia in Mice. J. Clin. Invest., 188(12): 3893-3903, 2008
[15]  Wen, Y, Pinto, J.R., Xu, Y., Wang, Y., Wang, Y., Gomes, A.V., Potter, J.D. and Kerrick, W.G.L. Functional Consequences of a Cardiac Troponin I (R145G) Mutation Associated with Hypertrophic Cardiomyopathy in Transgenic Mice. J. Biol. Chem., 283(29): 20484-20494, 2008
[16]  Landstrom, A.P.*, Parvatiyar, M.S.*, Pinto, J.R.*, Marquardt, M.L., Bos, J.M., Tester, D.J., Ommen, S.R., Potter, J.D. and Ackerman, M.J. Molecular and Functional Characterization of Novel Hypertrophic Cardiomyopathy Susceptibility Mutations in TNNC1-Encoded Troponin C. J. Mol. Cell Card., 45: 281-288, 2008 (*Co-first author)
[17]  Pinto, J.R., Veltri, T. and Sorenson, M.M. Modulation of Troponin C Affinity for the Thin Filament by Different Types of Crossbridges in Skinned Skeletal Muscle. Pflugers Archive, 456: 1177-1187, 2008
[18]  Pinto, J.R., Parvatiyar, M.S., Jones, M.A., Liang, J. and Potter, J.D. A Troponin T Mutation that Causes Infantile Restrictive Cardiomyopathy Increases Ca2+ Sensitivity of Force Development and Impairs the Inhibitory Properties of Troponin. J. Biol. Chem. 283: 2156-2166, 2008
[19]  Sousa, V.P., Pinto, J.R. and Sorenson, M.M. Ionic Interventions that Alter the Association of Troponin C C-domain with the Thin Filaments of Vertebrate Striated Muscle. Biochim. Biophys. Acta (Gen. Subj.) 1760: 272-282, 2006
[20]  Braga, C.A.C.A., Pinto, J.R., Valente, A.P., Silva, J.L., Sorenson, M.M., Foguel, D. and Suarez, M.C. Ca2+ and Mg2+ Binding to the Weak Sites of TnC C-domain Induces Exposure of a Large Hydrophobic Surface that Leads to Loss of TnC from the Thin Filament. Intl. J. Biochem. Cell Biol. 38: 110-122, 2006
[21]  Costa, L.T., Pinto, J.R., Moraes, M.B., Bastos, G.G., Sorenson, M.M., Bisch, P.M., and Weissmüller, G. Chemical Treatment of Mica for Atomic Force Microscopy Can Affect Biological Sample Conformation. Biophys. Chem. 109: 63-71, 2004