Nutritional Assessment of Wheat Biscuits and Fortified Wheat Biscuits with Citrus Peels Powders

Citrus peels, the waste by-products of citrus juices factories are reckoned valuable healthful functional food. The present investigation performed to assess nutritional status of wheat and fortified biscuits with citrus peels powders. Study included determination of gross chemical composition, caloric value , minerals (Fe, Mn, Cu, Ca, Mg, Na, K and P) of wheat and fortified biscuits with citrus peels powders. Likewise physical and sensory characteristics of control and 10% fortified wheat biscuits with the four studied citrus peels powders: Tangerine peel powder, Abo-Sora peel powder, Baladi orange peel powder, and Baladi lemon peel powder. The data revealed that 10% incorporation of citrus peels powders in wheat biscuits increased crude protein, crude fat contents as well as crude fiber, moisture contents and caloric value. However, it decreased carbohydrate content. The supplementation of control biscuits with 10% citrus peels powders of the four studied citrus enhanced the nutritive value. The data revealed that all 10% fortified biscuits with the four studied citrus peels powders improved all studied sensory characteristics in all the studied biscuits. Moreover, biscuits fortified with 10% citrus peel powders. Could be recommended for caloric reduced diets for obese, over-weight persons and diabetic persons.


Introduction
It is note-worthy to clarify that citrus peels: the waste by-product of the citrus factories are reckoned as a valuable functional food. So, citrus peels may provide a health benefit beyond the traditional nutrients they contain, as well as prevent diet-related diseases, e.g. metabolic syndrome, type II diabetes, coronary heat disease, obesity, hypertension, certain types of cancer, gastrointestinal diseases and osteoporosis (Block et al., 1992); Steinmetz and Potter (1996); Donalson (2004); and Youssef (2007).
On the other hand, pectin found in highest concentration in segment membrane of citrus fruits appears to be able to prevent prostate and other cancers by acting as mediator in cell communication, a factor known to reduce the likelihood of abnormal cell growth. Sour fruits as lemon appear to have the greatest effect (Liu et al., 2001) and Sugimura (2002).
Furthermore, the dietary fibers are the indigestible, fibrous part of citrus fruits are an essential part of our diet. Because of their water retaining properties, fibers help food pass through the gut faster and therefore have a laxative effect.
Fibers add bulk to the diet and fill up, making less likely to snack on fatty foods. Therefore, we need to eat fibers every day as part of a balanced diet (Youssef, 2007).
Moreover, pectins, the fiber in citrus peel are fermented in the intestines, producing short-chain fatty acids that prevent the growth of harmful bacteria. They also nourish the cells of the intestinal lining, making them more resistant to becoming cancerous (Donalson, 2004).
Furthermore, we can get soluble fibers from citrus peels, namely from oranges and grape fruit (Bobroff, 2002) and de Vries (2004). Archibald (2005) outlined the beneficial physiological role of dietary fiber in laxation, blood cholesterol and blood glucose attenuation. Sulamyn et al. (1990) reported the effect of different methods of extraction on the physico-chemical properties of Egyptian summer orange peel oils. Haggag (1992) carried out a pharmacognostical study of citrus aurantifolia swingle and citrus limonia osbeck (family: Rutaceae). She reported that the galactouronic acid content of lime pectin was higher than that of lemon pectin. Eisa (1995) studying the composition of lemon and mandarin peel essential oils and their utilization as anti-pathogenic bacteria agents found that mandarin and lemon peels contained 0.59% and 0.40% volatile oil; respectively. Mandarin peel oil was characterized by the presence of high concentration of gamma -terpinene, while lemon peel oil was identified by the presence of beta-pinene, geranyl acetate and methyl cinnamate. Kamel (1990) studied the effect of gamma irradiation on the total soluble solids, titratable acidity, vitamin C, total sugars, total free amino acids, total carotenoids, total phenols and volatile peel oil of navel and Valencia orange fruits. Abdel-Rauf (1997) carried out a study on the production and evaluation of some citrus low terpenes essential oils. Aumar (1997) studied the biochemical components of the essential oils of Balady orange and mandarin. Abdel-Galil (1998) carried out a study on the physicochemical properties of certain citrus peel volatile oils.
El-Shemy et al. (1990) reported the relation between irradiation doses and browning oxidative enzymes and phenol of grapefruits. The phenolic compounds and volatile oils of grapefruits were evaluated. Cohen et al. (1984)  This investigation was designed to produce fortified wheat biscuits with citrus peel powder, which has better nutritional value, available and relatively cheap and does not require any preparation efforts before using. The objective of this investigation was to study the gross chemical composition, caloric value, the mineral composition as well as physical characteristics and sensory quality attributes of wheat biscuits and wheat biscuits fortified with citrus peels powders.

Materials
5 kg wheat flour 72% extraction hard red winter were obtained from El-Haram Milling Company, Faesal, Giza in January 2011. Sugar powder, corn oil, sodium chloride, ammonium bicarbonate, and rose oil were purchased from Assiut local market in January 2011. 20 kg of each of the four studied citrus fruits were procured from Assiut University horticulture farm, namely: Baladi orange, Abo-Sora orange, Tangerine and Baladi lemon in January 2011.
The citrus fruits were peeled and the obtained peels were sun dried to complete dryness on wood trays. The dried peels were milled by hammer mill to produce citrus peels powders. The citrus peel powder was kept in glass containers at 4°C in the refrigerator till the analysis.

Biscuit Formula and Ingredients
Control biscuit dough was prepared according to the formula presented in table (1), (Saba, 1997). The supplemented biscuits with citrus peels powders were prepared using the same formula except for replacing the wheat flour with 10% of each of the four studied citrus peels powders.

Dough Preparation
Powdered sugar and corn oil were creamed in Braun Mixer with a flat beater for 2 minutes at 6 rpm. Water containing sodium chloride, ammonium bicarbonate and rose oil was added to the cream and mixed for 5 minutes at 125 rpm to obtain a homogenous cream. Thereafter flour was added slowly to the above cream and was mixed for 2 minutes at 60 rpm to obtain biscuit dough (Saba, 1997).

Preparation of Biscuit
The dough was sheeted to a thickness of about 3 mm using Atlas Brand rolling machine. The sheeted dough was cut into round shape using a 45 mm diameter cutter and baked on an aluminium tray in an electric oven at 180°C for 6 minutes. The biscuit was cooled for 30 minutes, packed in polyethylene bags stored under desiccation (Vatsala and Hardias Rao, 1991, and Manohar and Rao, 1997).

Preparation of Different Blends of Biscuits
Blends of biscuits were prepared using wheat flour 72% extraction rate as control or those which substituted with 10% of Tangerine peel powder, 10% Abo-Sora orange peel powder, 10% Baladi orange peel powder, and 10% Baladi lemon peel powder.

Physical Evaluation of Biscuits
Biscuits were evaluated for height (cm), width (cm), spread ratio and spread factor. Five biscuits were used for the evaluations from each of the five studied biscuits and averages were recorded. The spread ratio and spread factor were calculated according to Manohar and Rao (1997)

Sensory Evaluation of Biscuits:
Sensory evaluation for the color, texture, taste, odor and overall acceptability were done in order to determine consumer acceptability. A numerical hedonic scale ranging from 1 to 10 (1 is very bad and 10 for excellent) was used for sensory evaluation (Larmond, 1977). Ten experienced judges participated in the test.

Proximate Chemical Composition
Moisture, protein, crude fibers and ash contents were determined according to the method described in the AOAC (1997). Fat content was determined as the ether extract according to AOCS (1994). Total carbohydrates were calculated by difference according to Pellet and Sossy (1970). All determinations were performed in triplicates and the means were reported. The caloric value was calculated using values of 4 k.cal/g. of protein, 4 k.cal/g. of carbohydrate and 9 k.cal/g. of fat according to Livesy (1995).

Determination of Mineral Contents
The minerals contents i.e. (sodium, potassium, magnesium, calcium, iron, copper, manganese and phosphorus of wheat biscuits and fortified wheat biscuits with citrus peels powder were determined according to the methods described in AOAC (1997). The samples were wet acid digested using a nitric acid and perchloric acid mixture (HNO 3 , HCLO 4 , 2:1 v/v). The amounts of iron, copper, and manganese in the digested sample were determined using a GBC Atomic Absorption 906 A as described in AOAC (1997). Sodium and potassium were determined by flame photometer 410. Calcium and magnesium were determined using Double Beam Atomic Absorption. Phosphorus was determined according to the method described in A.O.A.C. (1997).

Physical Characteristics of Biscuits
Citrus peels had been considered as a functional food supplement in certain good products because they are reckoned as a good source of dietary fibers, volatile oils, phenolic compounds, minerals and vitamins.
The mean values of physical characteristics of wheat biscuit and fortified wheat biscuits with citrus peels powder are presented in Table (2) and Figures 1-5. The data recorded a gradual increment of spread ratio of all fortified biscuits with all the four studied citrus peels powders ranging from 2.72 to 2.92 for 10% Tangerine peel powder supplemented biscuits and 10% Abo-Sora orange peel powder supplemented biscuits. Considering the spread factor of control biscuits (100% wheat flour-72% extraction biscuits) as 100, results given in Table (2) indicated that it increased to 104.00, 111.00, 110.00 and 108.000; for 10% Tangerine peel powder supplemented biscuits, 10% Abo-Sora orange peel powder supplemented biscuits, 10% Baladi orange peel powder supplemented biscuits; and Baladi lemon peel powder supplemented biscuits; respectively.

Sensory Characteristics of Biscuits
The sensory characteristics of the studied wheat biscuits as influenced by the incorporation of 10% Tangerine peel powder, 10% Abo-Sora orange peel powder, 10% Baladi orange peel powder, and 10% Baladi lemon peel powder are outlined in table (3). The data revealed that all fortified biscuits with 10% of the four studied citrus peels powders improved all studied sensory characteristics in all the studied biscuit samples. However, the best scores of all studied sensory characteristics were recorded for 10% Baladi orange peel powder supplemented biscuits and 10% Abo-Sora orange peel powder supplemented biscuits.
Therefore, both 10% Baladi orange peel powder supplemented biscuits and 10% Abo-Sora orange peel powder supplemented biscuits could be recommended to be produced as biscuits with good quality acceptable sensory quality attributes. Likewise, on the basis of such result further analysis of gross chemical composition and mineral composition of the four fortified wheat biscuits with 10% of the four studied citrus peel powders were evaluated and were compared with the control biscuits (100% wheat flour -72% extraction biscuits).

Gross Chemical Composition of Biscuits
The mean value of gross chemical composition and caloric value of wheat biscuits and fortified wheat biscuits with citrus peel powders are given in table (4). The data revealed that 10% incorporation of citrus peels powders in wheat flour biscuits increased crude protein, crude fat contents as well as crude fiber, moisture contents and caloric value. However, such supplementation decreased carbohydrate content.

Minerals Composition of Biscuits
The mean values of minerals composition of wheat biscuits, and fortified wheat biscuits with citrus peel powders are outlined in table (5). The data revealed that 10% Abo-Sora orange peel powder supplemented wheat biscuits, and 10% Baladi lemon peel powder supplemented wheat biscuits had the highest Ca; and Na and K contents, respectively. Meanwhile, the latter 10% Baladi lemon peel recorded in addition the highest P content among all studied biscuits on dry weight basis. Such data coincide with Cohen et al. (1984), Sulamyn et al. (1990), and Youssef (2007).   In conclusion, the four studied wheat biscuits fortified with 10% citrus peel powders proved to be nutritious functional and healthful foods. Moreover, they could be recommended for caloric reduced diets for obese and over-weight persons especially 10% Tangerine peel powder supplemented wheat biscuits. Likewise, the rather relative low carbohydrate content in the four studied wheat biscuits fortified with 10% citrus peels powders could be recommended for the diet regimen of diabetic persons.